Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06225

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06220 and BWI76_RS06225 overlap by 4 nucleotidesBWI76_RS06225 and BWI76_RS06230 are separated by 294 nucleotides BWI76_RS06220: BWI76_RS06220 - exonuclease subunit SbcC, at 1,303,115 to 1,306,249 _RS06220 BWI76_RS06225: BWI76_RS06225 - exonuclease sbcCD subunit D, at 1,306,246 to 1,307,448 _RS06225 BWI76_RS06230: BWI76_RS06230 - DNA-binding response regulator, at 1,307,743 to 1,308,432 _RS06230 Position (kb) 1306 1307 1308Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1305.804 kb on - strand, within BWI76_RS06220at 1306.072 kb on - strandat 1306.072 kb on - strandat 1306.174 kb on + strandat 1306.296 kb on + strandat 1306.427 kb on - strand, within BWI76_RS06225at 1306.854 kb on - strand, within BWI76_RS06225at 1306.946 kb on - strand, within BWI76_RS06225at 1307.040 kb on - strand, within BWI76_RS06225at 1307.278 kb on + strand, within BWI76_RS06225at 1307.278 kb on + strand, within BWI76_RS06225at 1307.431 kb on + strandat 1307.432 kb on - strandat 1307.514 kb on - strandat 1307.514 kb on - strandat 1307.530 kb on + strandat 1307.540 kb on - strandat 1307.567 kb on + strandat 1307.575 kb on - strandat 1307.707 kb on + strandat 1307.707 kb on + strandat 1307.707 kb on + strandat 1307.855 kb on + strand, within BWI76_RS06230at 1307.865 kb on + strand, within BWI76_RS06230at 1307.933 kb on + strand, within BWI76_RS06230at 1308.435 kb on + strandat 1308.444 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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1,305,804 - BWI76_RS06220 0.86 -0.2
1,306,072 - -1.0
1,306,072 - +1.9
1,306,174 + +0.8
1,306,296 + +0.2
1,306,427 - BWI76_RS06225 0.15 -0.6
1,306,854 - BWI76_RS06225 0.51 +0.2
1,306,946 - BWI76_RS06225 0.58 -0.7
1,307,040 - BWI76_RS06225 0.66 +2.9
1,307,278 + BWI76_RS06225 0.86 +3.7
1,307,278 + BWI76_RS06225 0.86 +0.1
1,307,431 + -1.0
1,307,432 - +1.2
1,307,514 - +0.2
1,307,514 - +1.7
1,307,530 + -0.6
1,307,540 - -2.9
1,307,567 + +0.5
1,307,575 - -0.0
1,307,707 + +0.1
1,307,707 + -0.6
1,307,707 + +0.5
1,307,855 + BWI76_RS06230 0.16 +0.2
1,307,865 + BWI76_RS06230 0.18 -0.2
1,307,933 + BWI76_RS06230 0.28 -1.4
1,308,435 + +0.8
1,308,444 + +0.2

Or see this region's nucleotide sequence