Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04170 and BWI76_RS04175 are separated by 98 nucleotides BWI76_RS04175 and BWI76_RS04180 overlap by 60 nucleotides BWI76_RS04180 and BWI76_RS04185 are separated by 125 nucleotides
BWI76_RS04170: BWI76_RS04170 - trifunctional nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase/transcriptional regulator NadR, at 858,057 to 859,289
_RS04170
BWI76_RS04175: BWI76_RS04175 - energy-dependent translational throttle protein EttA, at 859,388 to 861,055
_RS04175
BWI76_RS04180: BWI76_RS04180 - hypothetical protein, at 860,996 to 861,203
_RS04180
BWI76_RS04185: BWI76_RS04185 - murein transglycosylase, at 861,329 to 863,266
_RS04185
Position (kb) 859
860
861
862 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 858.530 kb on - strand, within BWI76_RS04170 at 858.594 kb on - strand, within BWI76_RS04170 at 858.772 kb on - strand, within BWI76_RS04170 at 858.893 kb on + strand, within BWI76_RS04170 at 859.069 kb on + strand, within BWI76_RS04170 at 859.127 kb on - strand, within BWI76_RS04170 at 859.167 kb on + strand at 859.208 kb on - strand at 859.257 kb on - strand at 859.318 kb on + strand at 859.507 kb on + strand at 859.589 kb on + strand, within BWI76_RS04175 at 859.713 kb on + strand, within BWI76_RS04175 at 859.742 kb on - strand, within BWI76_RS04175 at 860.196 kb on + strand, within BWI76_RS04175 at 861.021 kb on - strand at 861.062 kb on - strand, within BWI76_RS04180 at 861.082 kb on + strand, within BWI76_RS04180 at 861.118 kb on + strand, within BWI76_RS04180 at 861.126 kb on - strand, within BWI76_RS04180 at 861.131 kb on - strand, within BWI76_RS04180 at 861.133 kb on + strand, within BWI76_RS04180 at 861.176 kb on + strand, within BWI76_RS04180 at 861.293 kb on + strand at 861.530 kb on - strand, within BWI76_RS04185 at 861.687 kb on + strand, within BWI76_RS04185 at 861.768 kb on - strand, within BWI76_RS04185 at 861.778 kb on - strand, within BWI76_RS04185 at 861.835 kb on + strand, within BWI76_RS04185 at 861.897 kb on - strand, within BWI76_RS04185 at 862.016 kb on + strand, within BWI76_RS04185
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 858,530 - BWI76_RS04170 0.38 -0.5 858,594 - BWI76_RS04170 0.44 -0.2 858,772 - BWI76_RS04170 0.58 -2.1 858,893 + BWI76_RS04170 0.68 +0.1 859,069 + BWI76_RS04170 0.82 -0.2 859,127 - BWI76_RS04170 0.87 +0.3 859,167 + -3.3 859,208 - -2.6 859,257 - -0.9 859,318 + -1.0 859,507 + +2.0 859,589 + BWI76_RS04175 0.12 -0.6 859,713 + BWI76_RS04175 0.19 +4.5 859,742 - BWI76_RS04175 0.21 +0.2 860,196 + BWI76_RS04175 0.48 -1.0 861,021 - +0.4 861,062 - BWI76_RS04180 0.32 -1.5 861,082 + BWI76_RS04180 0.41 -1.7 861,118 + BWI76_RS04180 0.59 -1.4 861,126 - BWI76_RS04180 0.62 +0.5 861,131 - BWI76_RS04180 0.65 -1.2 861,133 + BWI76_RS04180 0.66 -0.7 861,176 + BWI76_RS04180 0.87 -2.2 861,293 + +2.2 861,530 - BWI76_RS04185 0.10 -0.1 861,687 + BWI76_RS04185 0.18 +0.1 861,768 - BWI76_RS04185 0.23 -1.1 861,778 - BWI76_RS04185 0.23 -0.3 861,835 + BWI76_RS04185 0.26 -0.8 861,897 - BWI76_RS04185 0.29 +1.0 862,016 + BWI76_RS04185 0.35 -3.0
Or see this region's nucleotide sequence