Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02665 and BWI76_RS02670 are separated by 4 nucleotides BWI76_RS02670 and BWI76_RS02675 are separated by 41 nucleotides BWI76_RS02675 and BWI76_RS02680 are separated by 146 nucleotides BWI76_RS02680 and BWI76_RS02685 are separated by 4 nucleotides BWI76_RS02685 and BWI76_RS02690 are separated by 139 nucleotides BWI76_RS02690 and BWI76_RS02695 overlap by 69 nucleotides
BWI76_RS02665: BWI76_RS02665 - primosomal replication protein N, at 542,874 to 543,188
_RS02665
BWI76_RS02670: BWI76_RS02670 - 30S ribosomal protein S18, at 543,193 to 543,420
_RS02670
BWI76_RS02675: BWI76_RS02675 - 50S ribosomal protein L9, at 543,462 to 543,911
_RS02675
BWI76_RS02680: BWI76_RS02680 - EamA family transporter, at 544,058 to 545,011
_RS02680
BWI76_RS02685: BWI76_RS02685 - opacity-associated protein, at 545,016 to 545,675
_RS02685
BWI76_RS02690: BWI76_RS02690 - hypothetical protein, at 545,815 to 546,012
_RS02690
BWI76_RS02695: BWI76_RS02695 - peptidyl-prolyl cis-trans isomerase, at 545,944 to 546,564
_RS02695
Position (kb) 544
545
546 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 543.723 kb on + strand, within BWI76_RS02675 at 544.109 kb on + strand at 544.262 kb on + strand, within BWI76_RS02680 at 544.275 kb on - strand, within BWI76_RS02680 at 544.367 kb on - strand, within BWI76_RS02680 at 544.880 kb on + strand, within BWI76_RS02680 at 545.124 kb on + strand, within BWI76_RS02685 at 545.147 kb on + strand, within BWI76_RS02685 at 545.150 kb on - strand, within BWI76_RS02685 at 545.207 kb on + strand, within BWI76_RS02685 at 545.224 kb on + strand, within BWI76_RS02685 at 545.265 kb on - strand, within BWI76_RS02685 at 545.499 kb on + strand, within BWI76_RS02685 at 545.507 kb on - strand, within BWI76_RS02685 at 545.713 kb on + strand at 545.776 kb on - strand at 545.776 kb on - strand at 545.782 kb on + strand at 545.867 kb on + strand, within BWI76_RS02690 at 545.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 543,723 + BWI76_RS02675 0.58 -1.8 544,109 + +0.2 544,262 + BWI76_RS02680 0.21 -0.0 544,275 - BWI76_RS02680 0.23 -0.6 544,367 - BWI76_RS02680 0.32 -1.7 544,880 + BWI76_RS02680 0.86 -1.1 545,124 + BWI76_RS02685 0.16 -0.6 545,147 + BWI76_RS02685 0.20 +9.2 545,150 - BWI76_RS02685 0.20 -0.0 545,207 + BWI76_RS02685 0.29 -3.7 545,224 + BWI76_RS02685 0.32 -0.3 545,265 - BWI76_RS02685 0.38 -0.9 545,499 + BWI76_RS02685 0.73 -0.2 545,507 - BWI76_RS02685 0.74 -2.0 545,713 + -0.1 545,776 - -2.1 545,776 - +2.3 545,782 + +0.7 545,867 + BWI76_RS02690 0.26 -0.2 545,966 + -0.7
Or see this region's nucleotide sequence