Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02230 and BWI76_RS02235 are separated by 204 nucleotides BWI76_RS02235 and BWI76_RS02240 are separated by 160 nucleotides BWI76_RS02240 and BWI76_RS02245 overlap by 6 nucleotides BWI76_RS02245 and BWI76_RS02250 are separated by 330 nucleotides
BWI76_RS02230: BWI76_RS02230 - phosphonate metabolism transcriptional regulator PhnF, at 460,455 to 461,180
_RS02230
BWI76_RS02235: BWI76_RS02235 - VOC family protein, at 461,385 to 461,819
_RS02235
BWI76_RS02240: BWI76_RS02240 - alkylphosphonate utilization operon protein PhnA, at 461,980 to 462,315
_RS02240
BWI76_RS02245: BWI76_RS02245 - hypothetical protein, at 462,310 to 462,525
_RS02245
BWI76_RS02250: BWI76_RS02250 - proline/betaine transporter, at 462,856 to 464,358
_RS02250
Position (kb) 461
462
463 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 461.027 kb on + strand, within BWI76_RS02230 at 461.027 kb on + strand, within BWI76_RS02230 at 461.035 kb on - strand, within BWI76_RS02230 at 461.035 kb on - strand, within BWI76_RS02230 at 461.196 kb on + strand at 461.409 kb on - strand at 461.556 kb on + strand, within BWI76_RS02235 at 461.634 kb on + strand, within BWI76_RS02235 at 461.664 kb on - strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.751 kb on - strand, within BWI76_RS02235 at 461.774 kb on + strand, within BWI76_RS02235 at 461.777 kb on - strand at 461.800 kb on + strand at 461.809 kb on + strand at 461.815 kb on + strand at 461.824 kb on - strand at 461.828 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.853 kb on + strand at 461.853 kb on + strand at 461.853 kb on + strand at 461.854 kb on - strand at 461.861 kb on - strand at 461.861 kb on - strand at 461.895 kb on + strand at 461.895 kb on + strand at 461.900 kb on + strand at 461.901 kb on - strand at 461.908 kb on - strand at 461.914 kb on + strand at 461.919 kb on + strand at 461.919 kb on + strand at 461.921 kb on + strand at 461.921 kb on + strand at 461.927 kb on - strand at 462.021 kb on - strand, within BWI76_RS02240 at 462.040 kb on - strand, within BWI76_RS02240 at 462.055 kb on + strand at 462.056 kb on + strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.073 kb on + strand, within BWI76_RS02240 at 462.102 kb on + strand, within BWI76_RS02240 at 462.112 kb on + strand, within BWI76_RS02240 at 462.112 kb on + strand, within BWI76_RS02240 at 462.113 kb on + strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.121 kb on - strand, within BWI76_RS02240 at 462.121 kb on - strand, within BWI76_RS02240 at 462.144 kb on + strand, within BWI76_RS02240 at 462.202 kb on + strand, within BWI76_RS02240 at 462.204 kb on + strand, within BWI76_RS02240 at 462.205 kb on + strand, within BWI76_RS02240 at 462.212 kb on + strand, within BWI76_RS02240 at 462.213 kb on + strand, within BWI76_RS02240 at 462.218 kb on + strand, within BWI76_RS02240 at 462.220 kb on - strand, within BWI76_RS02240 at 462.221 kb on - strand, within BWI76_RS02240 at 462.223 kb on + strand, within BWI76_RS02240 at 462.226 kb on - strand, within BWI76_RS02240 at 462.226 kb on - strand at 462.228 kb on + strand, within BWI76_RS02240 at 462.260 kb on - strand, within BWI76_RS02240 at 462.273 kb on - strand, within BWI76_RS02240 at 462.274 kb on + strand, within BWI76_RS02240 at 462.299 kb on + strand at 462.317 kb on - strand at 462.343 kb on - strand, within BWI76_RS02245 at 462.343 kb on - strand, within BWI76_RS02245 at 462.376 kb on - strand, within BWI76_RS02245 at 462.380 kb on + strand, within BWI76_RS02245 at 462.382 kb on - strand, within BWI76_RS02245 at 462.435 kb on + strand, within BWI76_RS02245 at 462.443 kb on - strand, within BWI76_RS02245 at 462.443 kb on - strand, within BWI76_RS02245 at 462.443 kb on - strand, within BWI76_RS02245 at 462.514 kb on + strand at 462.526 kb on + strand at 462.558 kb on - strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.613 kb on + strand at 462.613 kb on + strand at 462.656 kb on - strand at 462.664 kb on + strand at 462.680 kb on + strand at 462.680 kb on + strand at 462.683 kb on - strand at 462.685 kb on + strand at 462.685 kb on + strand at 462.685 kb on + strand at 462.685 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.693 kb on + strand at 462.693 kb on - strand at 462.693 kb on - strand at 462.693 kb on - strand at 462.693 kb on - strand at 462.698 kb on - strand at 462.698 kb on - strand at 462.699 kb on + strand at 462.706 kb on + strand at 462.708 kb on + strand at 462.714 kb on - strand at 462.718 kb on + strand at 462.727 kb on - strand at 462.727 kb on - strand at 462.729 kb on + strand at 462.729 kb on + strand at 462.729 kb on + strand at 462.733 kb on + strand at 462.733 kb on + strand at 462.734 kb on + strand at 462.737 kb on - strand at 462.737 kb on - strand at 462.746 kb on - strand at 462.749 kb on + strand at 462.755 kb on + strand at 462.760 kb on + strand at 462.763 kb on - strand at 462.765 kb on + strand at 462.793 kb on - strand at 462.802 kb on - strand at 462.813 kb on + strand at 462.841 kb on - strand at 462.889 kb on + strand at 462.951 kb on + strand at 462.951 kb on + strand at 463.008 kb on + strand, within BWI76_RS02250 at 463.023 kb on + strand, within BWI76_RS02250 at 463.050 kb on - strand, within BWI76_RS02250 at 463.050 kb on - strand, within BWI76_RS02250 at 463.059 kb on + strand, within BWI76_RS02250 at 463.117 kb on + strand, within BWI76_RS02250 at 463.259 kb on - strand, within BWI76_RS02250 at 463.276 kb on - strand, within BWI76_RS02250 at 463.285 kb on + strand, within BWI76_RS02250 at 463.285 kb on + strand, within BWI76_RS02250 at 463.293 kb on - strand, within BWI76_RS02250
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 461,027 + BWI76_RS02230 0.79 +0.6 461,027 + BWI76_RS02230 0.79 -0.5 461,035 - BWI76_RS02230 0.80 +0.1 461,035 - BWI76_RS02230 0.80 +5.9 461,196 + -1.2 461,409 - -0.4 461,556 + BWI76_RS02235 0.39 +0.2 461,634 + BWI76_RS02235 0.57 +0.1 461,664 - BWI76_RS02235 0.64 +0.4 461,738 + BWI76_RS02235 0.81 +2.3 461,738 + BWI76_RS02235 0.81 +0.2 461,738 + BWI76_RS02235 0.81 +0.3 461,746 - BWI76_RS02235 0.83 -0.8 461,746 - BWI76_RS02235 0.83 -0.4 461,746 - BWI76_RS02235 0.83 +1.8 461,746 - BWI76_RS02235 0.83 +0.4 461,751 - BWI76_RS02235 0.84 +5.2 461,774 + BWI76_RS02235 0.89 +1.8 461,777 - -0.6 461,800 + +3.0 461,809 + +1.3 461,815 + -0.4 461,824 - -0.2 461,828 - -0.5 461,830 - +1.5 461,830 - -2.1 461,830 - -0.2 461,830 - +0.5 461,853 + +0.2 461,853 + -0.5 461,853 + +4.2 461,854 - -0.1 461,861 - +1.0 461,861 - +0.3 461,895 + +2.9 461,895 + +1.0 461,900 + -0.8 461,901 - +0.8 461,908 - +0.4 461,914 + -1.4 461,919 + +1.5 461,919 + -1.4 461,921 + -0.5 461,921 + +0.6 461,927 - -1.3 462,021 - BWI76_RS02240 0.12 +0.8 462,040 - BWI76_RS02240 0.18 +0.3 462,055 + +0.8 462,056 + BWI76_RS02240 0.23 +0.4 462,064 - BWI76_RS02240 0.25 -0.3 462,064 - BWI76_RS02240 0.25 +0.1 462,064 - BWI76_RS02240 0.25 -0.7 462,064 - BWI76_RS02240 0.25 +0.1 462,064 - BWI76_RS02240 0.25 -0.4 462,064 - BWI76_RS02240 0.25 +0.2 462,064 - BWI76_RS02240 0.25 +5.7 462,073 + BWI76_RS02240 0.28 +0.5 462,102 + BWI76_RS02240 0.36 -0.6 462,112 + BWI76_RS02240 0.39 +1.8 462,112 + BWI76_RS02240 0.39 +0.4 462,113 + BWI76_RS02240 0.40 +1.6 462,120 - BWI76_RS02240 0.42 -0.9 462,120 - BWI76_RS02240 0.42 -1.1 462,120 - BWI76_RS02240 0.42 -3.5 462,121 - BWI76_RS02240 0.42 -0.4 462,121 - BWI76_RS02240 0.42 +0.2 462,144 + BWI76_RS02240 0.49 +1.5 462,202 + BWI76_RS02240 0.66 -1.5 462,204 + BWI76_RS02240 0.67 -2.5 462,205 + BWI76_RS02240 0.67 +0.8 462,212 + BWI76_RS02240 0.69 -0.4 462,213 + BWI76_RS02240 0.69 +1.6 462,218 + BWI76_RS02240 0.71 -0.7 462,220 - BWI76_RS02240 0.71 +0.9 462,221 - BWI76_RS02240 0.72 +0.8 462,223 + BWI76_RS02240 0.72 -0.1 462,226 - BWI76_RS02240 0.73 -0.1 462,226 - +1.2 462,228 + BWI76_RS02240 0.74 -0.2 462,260 - BWI76_RS02240 0.83 -1.4 462,273 - BWI76_RS02240 0.87 -1.0 462,274 + BWI76_RS02240 0.88 -1.2 462,299 + +0.4 462,317 - -1.0 462,343 - BWI76_RS02245 0.15 +2.0 462,343 - BWI76_RS02245 0.15 -0.1 462,376 - BWI76_RS02245 0.31 +0.1 462,380 + BWI76_RS02245 0.32 -0.7 462,382 - BWI76_RS02245 0.33 +1.5 462,435 + BWI76_RS02245 0.58 -1.3 462,443 - BWI76_RS02245 0.62 -0.3 462,443 - BWI76_RS02245 0.62 -0.4 462,443 - BWI76_RS02245 0.62 -0.3 462,514 + +1.3 462,526 + +0.6 462,558 - -0.2 462,566 + -0.5 462,566 + +0.5 462,566 + -0.6 462,566 + -0.9 462,566 + -1.1 462,566 + -0.5 462,566 + +0.4 462,566 + +0.0 462,566 + +3.8 462,566 + -0.4 462,566 + -1.2 462,566 + -1.1 462,566 + -0.1 462,566 + -0.8 462,566 + +1.2 462,566 + -0.4 462,566 + -1.3 462,566 + +0.5 462,566 + +0.5 462,574 - -1.6 462,574 - +1.5 462,574 - +0.2 462,574 - -2.6 462,574 - -0.7 462,574 - +0.2 462,574 - -0.1 462,574 - -0.0 462,574 - +0.7 462,574 - -3.0 462,574 - +0.5 462,574 - -0.9 462,574 - -1.4 462,574 - -0.9 462,574 - -0.9 462,574 - -2.7 462,613 + -0.3 462,613 + -0.3 462,656 - -1.4 462,664 + +0.3 462,680 + +0.4 462,680 + -2.9 462,683 - +0.1 462,685 + +0.4 462,685 + +0.5 462,685 + -1.7 462,685 + +1.0 462,690 + +0.8 462,690 + +0.0 462,690 + +2.8 462,690 + -1.5 462,690 + +0.3 462,690 + +0.7 462,690 + -0.1 462,690 + -0.3 462,690 + -0.1 462,690 + +6.4 462,690 + +1.8 462,693 + +6.5 462,693 - +0.5 462,693 - -0.4 462,693 - -1.1 462,693 - -1.7 462,698 - -0.5 462,698 - -0.4 462,699 + -0.2 462,706 + -0.4 462,708 + +0.5 462,714 - +1.9 462,718 + +2.0 462,727 - +0.2 462,727 - -0.9 462,729 + +0.2 462,729 + -0.5 462,729 + +0.3 462,733 + -0.6 462,733 + -0.2 462,734 + -0.2 462,737 - +0.0 462,737 - -0.3 462,746 - -1.9 462,749 + +0.8 462,755 + +2.1 462,760 + -0.6 462,763 - -1.1 462,765 + +0.8 462,793 - +0.4 462,802 - +0.2 462,813 + +0.0 462,841 - +1.2 462,889 + -0.4 462,951 + +0.6 462,951 + -0.6 463,008 + BWI76_RS02250 0.10 -1.2 463,023 + BWI76_RS02250 0.11 -1.6 463,050 - BWI76_RS02250 0.13 +0.3 463,050 - BWI76_RS02250 0.13 -1.0 463,059 + BWI76_RS02250 0.14 -0.8 463,117 + BWI76_RS02250 0.17 +0.2 463,259 - BWI76_RS02250 0.27 +1.8 463,276 - BWI76_RS02250 0.28 +0.6 463,285 + BWI76_RS02250 0.29 -1.5 463,285 + BWI76_RS02250 0.29 -4.1 463,293 - BWI76_RS02250 0.29 +0.2
Or see this region's nucleotide sequence