Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01750 and BWI76_RS01755 are separated by 348 nucleotides BWI76_RS01755 and BWI76_RS01760 overlap by 4 nucleotides BWI76_RS01760 and BWI76_RS01765 are separated by 185 nucleotides
BWI76_RS01750: BWI76_RS01750 - Cro/Cl family transcriptional regulator, at 357,467 to 358,414
_RS01750
BWI76_RS01755: BWI76_RS01755 - 23S rRNA pseudouridine synthase F, at 358,763 to 359,635
_RS01755
BWI76_RS01760: BWI76_RS01760 - hypothetical protein, at 359,632 to 359,904
_RS01760
BWI76_RS01765: BWI76_RS01765 - hypothetical protein, at 360,090 to 361,031
_RS01765
Position (kb) 358
359
360 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 357.788 kb on - strand, within BWI76_RS01750 at 357.796 kb on + strand, within BWI76_RS01750 at 357.797 kb on + strand, within BWI76_RS01750 at 357.935 kb on + strand, within BWI76_RS01750 at 358.035 kb on + strand, within BWI76_RS01750 at 358.134 kb on + strand, within BWI76_RS01750 at 358.164 kb on - strand, within BWI76_RS01750 at 358.184 kb on - strand, within BWI76_RS01750 at 358.216 kb on + strand, within BWI76_RS01750 at 358.255 kb on - strand, within BWI76_RS01750 at 358.281 kb on - strand, within BWI76_RS01750 at 358.347 kb on + strand at 358.347 kb on + strand at 358.347 kb on + strand at 358.355 kb on - strand at 358.355 kb on - strand at 358.365 kb on - strand at 358.396 kb on - strand at 358.438 kb on - strand at 358.547 kb on + strand at 358.568 kb on - strand at 358.578 kb on + strand at 358.586 kb on - strand at 358.647 kb on - strand at 358.654 kb on + strand at 358.688 kb on + strand at 358.723 kb on + strand at 358.737 kb on - strand at 358.825 kb on + strand at 358.825 kb on + strand at 358.833 kb on - strand at 358.833 kb on - strand at 358.884 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.967 kb on - strand, within BWI76_RS01755 at 358.978 kb on + strand, within BWI76_RS01755 at 359.091 kb on + strand, within BWI76_RS01755 at 359.191 kb on + strand, within BWI76_RS01755 at 359.252 kb on - strand, within BWI76_RS01755 at 359.896 kb on - strand at 359.900 kb on + strand at 359.922 kb on + strand at 359.930 kb on - strand at 359.943 kb on - strand at 360.027 kb on - strand at 360.134 kb on + strand at 360.339 kb on - strand, within BWI76_RS01765 at 360.349 kb on + strand, within BWI76_RS01765 at 360.367 kb on + strand, within BWI76_RS01765 at 360.417 kb on - strand, within BWI76_RS01765 at 360.518 kb on + strand, within BWI76_RS01765 at 360.527 kb on - strand, within BWI76_RS01765 at 360.553 kb on + strand, within BWI76_RS01765 at 360.561 kb on - strand, within BWI76_RS01765 at 360.561 kb on - strand, within BWI76_RS01765
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 357,788 - BWI76_RS01750 0.34 -1.8 357,796 + BWI76_RS01750 0.35 -1.4 357,797 + BWI76_RS01750 0.35 +0.5 357,935 + BWI76_RS01750 0.49 +0.3 358,035 + BWI76_RS01750 0.60 +1.2 358,134 + BWI76_RS01750 0.70 +0.1 358,164 - BWI76_RS01750 0.74 -0.4 358,184 - BWI76_RS01750 0.76 +0.7 358,216 + BWI76_RS01750 0.79 -1.4 358,255 - BWI76_RS01750 0.83 -0.2 358,281 - BWI76_RS01750 0.86 -0.6 358,347 + -0.1 358,347 + -1.0 358,347 + -1.2 358,355 - +0.8 358,355 - -2.3 358,365 - +0.3 358,396 - -1.6 358,438 - -1.2 358,547 + -1.4 358,568 - -0.0 358,578 + -0.1 358,586 - -0.2 358,647 - -1.4 358,654 + -0.9 358,688 + +1.3 358,723 + +0.2 358,737 - -0.8 358,825 + +0.5 358,825 + +1.5 358,833 - -2.4 358,833 - +0.9 358,884 + BWI76_RS01755 0.14 +0.3 358,897 + BWI76_RS01755 0.15 -0.9 358,897 + BWI76_RS01755 0.15 +1.5 358,897 + BWI76_RS01755 0.15 +8.4 358,897 + BWI76_RS01755 0.15 -0.1 358,967 - BWI76_RS01755 0.23 +0.9 358,978 + BWI76_RS01755 0.25 -1.6 359,091 + BWI76_RS01755 0.38 -0.5 359,191 + BWI76_RS01755 0.49 +0.4 359,252 - BWI76_RS01755 0.56 +0.7 359,896 - -1.5 359,900 + -0.2 359,922 + -1.1 359,930 - +0.2 359,943 - -0.7 360,027 - -2.2 360,134 + +0.1 360,339 - BWI76_RS01765 0.26 -0.3 360,349 + BWI76_RS01765 0.27 -0.3 360,367 + BWI76_RS01765 0.29 -1.1 360,417 - BWI76_RS01765 0.35 +0.1 360,518 + BWI76_RS01765 0.45 +0.5 360,527 - BWI76_RS01765 0.46 +0.3 360,553 + BWI76_RS01765 0.49 -1.3 360,561 - BWI76_RS01765 0.50 -0.3 360,561 - BWI76_RS01765 0.50 +0.2
Or see this region's nucleotide sequence