Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01200 and BWI76_RS01205 overlap by 4 nucleotides BWI76_RS01205 and BWI76_RS01210 overlap by 4 nucleotides BWI76_RS01210 and BWI76_RS01215 overlap by 1 nucleotides BWI76_RS01215 and BWI76_RS01220 are separated by 77 nucleotides
BWI76_RS01200: BWI76_RS01200 - diaminopimelate epimerase, at 245,360 to 246,184
_RS01200
BWI76_RS01205: BWI76_RS01205 - DUF484 family protein, at 246,181 to 246,888
_RS01205
BWI76_RS01210: BWI76_RS01210 - tyrosine recombinase XerC, at 246,885 to 247,787
_RS01210
BWI76_RS01215: BWI76_RS01215 - flavin mononucleotide phosphatase, at 247,787 to 248,503
_RS01215
BWI76_RS01220: BWI76_RS01220 - DNA-dependent helicase II, at 248,581 to 250,743
_RS01220
Position (kb) 246
247
248 Strain fitness (log2 ratio) -2
-1
0
1
2 at 246.061 kb on - strand, within BWI76_RS01200 at 246.133 kb on + strand at 246.133 kb on + strand at 246.133 kb on + strand at 246.233 kb on + strand at 246.238 kb on + strand at 246.744 kb on + strand, within BWI76_RS01205 at 246.900 kb on - strand at 247.091 kb on + strand, within BWI76_RS01210 at 247.448 kb on + strand, within BWI76_RS01210 at 247.460 kb on + strand, within BWI76_RS01210 at 247.835 kb on - strand at 247.857 kb on + strand at 247.916 kb on - strand, within BWI76_RS01215
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 246,061 - BWI76_RS01200 0.85 +1.8 246,133 + +0.0 246,133 + -2.0 246,133 + -1.7 246,233 + +1.5 246,238 + -0.5 246,744 + BWI76_RS01205 0.80 -1.1 246,900 - -1.1 247,091 + BWI76_RS01210 0.23 -0.6 247,448 + BWI76_RS01210 0.62 +0.3 247,460 + BWI76_RS01210 0.64 +0.0 247,835 - +0.4 247,857 + +0.5 247,916 - BWI76_RS01215 0.18 +0.3
Or see this region's nucleotide sequence