Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00315 and BWI76_RS00320 are separated by 58 nucleotides BWI76_RS00320 and BWI76_RS00325 are separated by 124 nucleotides BWI76_RS00325 and BWI76_RS00330 overlap by 4 nucleotides BWI76_RS00330 and BWI76_RS00335 are separated by 71 nucleotides BWI76_RS00335 and BWI76_RS00340 are separated by 75 nucleotides
BWI76_RS00315: BWI76_RS00315 - 23S ribosomal RNA, at 66,486 to 69,413
_RS00315
BWI76_RS00320: BWI76_RS00320 - 5S ribosomal RNA, at 69,472 to 69,587
_RS00320
BWI76_RS00325: BWI76_RS00325 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 69,712 to 70,218
_RS00325
BWI76_RS00330: BWI76_RS00330 - molybdenum cofactor guanylyltransferase, at 70,215 to 70,796
_RS00330
BWI76_RS00335: BWI76_RS00335 - hypothetical protein, at 70,868 to 71,137
_RS00335
BWI76_RS00340: BWI76_RS00340 - stress response kinase A, at 71,213 to 72,199
_RS00340
Position (kb) 70
71 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 69.863 kb on - strand, within BWI76_RS00325 at 69.926 kb on - strand, within BWI76_RS00325 at 70.056 kb on + strand, within BWI76_RS00325 at 70.124 kb on - strand, within BWI76_RS00325 at 70.364 kb on - strand, within BWI76_RS00330 at 70.386 kb on + strand, within BWI76_RS00330 at 70.525 kb on + strand, within BWI76_RS00330 at 70.791 kb on - strand at 70.829 kb on - strand at 70.835 kb on + strand at 70.844 kb on - strand at 70.913 kb on + strand, within BWI76_RS00335 at 71.051 kb on - strand, within BWI76_RS00335 at 71.115 kb on - strand at 71.188 kb on + strand at 71.224 kb on + strand at 71.232 kb on - strand at 71.303 kb on - strand at 71.317 kb on - strand, within BWI76_RS00340 at 71.529 kb on + strand, within BWI76_RS00340 at 71.637 kb on - strand, within BWI76_RS00340
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 69,863 - BWI76_RS00325 0.30 -0.4 69,926 - BWI76_RS00325 0.42 +0.1 70,056 + BWI76_RS00325 0.68 +3.4 70,124 - BWI76_RS00325 0.81 +0.2 70,364 - BWI76_RS00330 0.26 +0.6 70,386 + BWI76_RS00330 0.29 +1.2 70,525 + BWI76_RS00330 0.53 +1.7 70,791 - -0.1 70,829 - -0.5 70,835 + -0.0 70,844 - -2.4 70,913 + BWI76_RS00335 0.17 -0.1 71,051 - BWI76_RS00335 0.68 -1.6 71,115 - +0.9 71,188 + +1.4 71,224 + +1.1 71,232 - -1.3 71,303 - -0.5 71,317 - BWI76_RS00340 0.11 +1.0 71,529 + BWI76_RS00340 0.32 +0.3 71,637 - BWI76_RS00340 0.43 +0.9
Or see this region's nucleotide sequence