Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27540 and BWI76_RS27545 are separated by 456 nucleotides BWI76_RS27545 and BWI76_RS27550 are separated by 159 nucleotides BWI76_RS27550 and BWI76_RS27555 overlap by 20 nucleotides
BWI76_RS27540: BWI76_RS27540 - glycoside hydrolase, at 5,716,436 to 5,717,815
_RS27540
BWI76_RS27545: BWI76_RS27545 - sugar isomerase, at 5,718,272 to 5,719,003
_RS27545
BWI76_RS27550: BWI76_RS27550 - hypothetical protein, at 5,719,163 to 5,719,837
_RS27550
BWI76_RS27555: BWI76_RS27555 - putative sugar kinase/transcriptional regulator, ATPase domain protein, at 5,719,818 to 5,720,750
_RS27555
Position (kb) 5718
5719
5720 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5717.315 kb on + strand, within BWI76_RS27540 at 5717.316 kb on + strand, within BWI76_RS27540 at 5717.405 kb on + strand, within BWI76_RS27540 at 5717.412 kb on + strand, within BWI76_RS27540 at 5717.420 kb on - strand, within BWI76_RS27540 at 5717.422 kb on + strand, within BWI76_RS27540 at 5717.472 kb on + strand, within BWI76_RS27540 at 5717.535 kb on - strand, within BWI76_RS27540 at 5717.574 kb on + strand, within BWI76_RS27540 at 5717.584 kb on + strand, within BWI76_RS27540 at 5717.592 kb on - strand, within BWI76_RS27540 at 5717.620 kb on + strand, within BWI76_RS27540 at 5717.652 kb on + strand, within BWI76_RS27540 at 5717.908 kb on + strand at 5717.956 kb on + strand at 5717.964 kb on + strand at 5717.972 kb on - strand at 5717.974 kb on + strand at 5718.007 kb on + strand at 5718.015 kb on - strand at 5718.044 kb on + strand at 5718.096 kb on - strand at 5718.096 kb on - strand at 5718.469 kb on - strand, within BWI76_RS27545 at 5718.508 kb on - strand, within BWI76_RS27545 at 5718.561 kb on + strand, within BWI76_RS27545 at 5718.571 kb on - strand, within BWI76_RS27545 at 5718.774 kb on + strand, within BWI76_RS27545 at 5718.775 kb on + strand, within BWI76_RS27545 at 5718.881 kb on - strand, within BWI76_RS27545 at 5718.881 kb on - strand, within BWI76_RS27545 at 5718.902 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5719.020 kb on - strand at 5719.150 kb on + strand at 5719.150 kb on + strand at 5719.151 kb on + strand at 5719.169 kb on + strand at 5719.176 kb on - strand at 5719.177 kb on - strand at 5719.177 kb on - strand at 5719.466 kb on - strand, within BWI76_RS27550 at 5719.481 kb on - strand, within BWI76_RS27550 at 5719.580 kb on + strand, within BWI76_RS27550 at 5719.580 kb on + strand, within BWI76_RS27550 at 5719.590 kb on - strand, within BWI76_RS27550 at 5719.590 kb on - strand, within BWI76_RS27550 at 5719.789 kb on - strand at 5719.796 kb on - strand at 5719.992 kb on - strand, within BWI76_RS27555
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,717,315 + BWI76_RS27540 0.64 +0.7 5,717,316 + BWI76_RS27540 0.64 +0.1 5,717,405 + BWI76_RS27540 0.70 +1.3 5,717,412 + BWI76_RS27540 0.71 +1.1 5,717,420 - BWI76_RS27540 0.71 -0.9 5,717,422 + BWI76_RS27540 0.71 -0.9 5,717,472 + BWI76_RS27540 0.75 -0.4 5,717,535 - BWI76_RS27540 0.80 -0.7 5,717,574 + BWI76_RS27540 0.82 +1.1 5,717,584 + BWI76_RS27540 0.83 -0.1 5,717,592 - BWI76_RS27540 0.84 +0.2 5,717,620 + BWI76_RS27540 0.86 -0.4 5,717,652 + BWI76_RS27540 0.88 -0.2 5,717,908 + +0.6 5,717,956 + +0.4 5,717,964 + -0.1 5,717,972 - -0.7 5,717,974 + -0.8 5,718,007 + -0.3 5,718,015 - -0.3 5,718,044 + +0.8 5,718,096 - -0.1 5,718,096 - +0.4 5,718,469 - BWI76_RS27545 0.27 -1.0 5,718,508 - BWI76_RS27545 0.32 +0.1 5,718,561 + BWI76_RS27545 0.39 +1.2 5,718,571 - BWI76_RS27545 0.41 +0.1 5,718,774 + BWI76_RS27545 0.69 -0.7 5,718,775 + BWI76_RS27545 0.69 +4.3 5,718,881 - BWI76_RS27545 0.83 -0.9 5,718,881 - BWI76_RS27545 0.83 +2.1 5,718,902 - BWI76_RS27545 0.86 +0.5 5,718,927 - BWI76_RS27545 0.89 +3.7 5,718,927 - BWI76_RS27545 0.89 +0.3 5,718,927 - BWI76_RS27545 0.89 -0.4 5,718,927 - BWI76_RS27545 0.89 -1.0 5,719,020 - -0.0 5,719,150 + +0.4 5,719,150 + -2.0 5,719,151 + -1.5 5,719,169 + -0.1 5,719,176 - -0.2 5,719,177 - -0.3 5,719,177 - +1.6 5,719,466 - BWI76_RS27550 0.45 -1.1 5,719,481 - BWI76_RS27550 0.47 +0.2 5,719,580 + BWI76_RS27550 0.62 -0.3 5,719,580 + BWI76_RS27550 0.62 -0.2 5,719,590 - BWI76_RS27550 0.63 +3.1 5,719,590 - BWI76_RS27550 0.63 +2.1 5,719,789 - -0.9 5,719,796 - -0.4 5,719,992 - BWI76_RS27555 0.19 +1.7
Or see this region's nucleotide sequence