Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15675 and BWI76_RS15680 are separated by 111 nucleotides BWI76_RS15680 and BWI76_RS15685 are separated by 85 nucleotides BWI76_RS15685 and BWI76_RS15690 overlap by 50 nucleotides BWI76_RS15690 and BWI76_RS15695 overlap by 4 nucleotides BWI76_RS15695 and BWI76_RS15700 overlap by 4 nucleotides BWI76_RS15700 and BWI76_RS15705 overlap by 4 nucleotides
BWI76_RS15675: BWI76_RS15675 - hypothetical protein, at 3,275,020 to 3,275,265
_RS15675
BWI76_RS15680: BWI76_RS15680 - hypothetical protein, at 3,275,377 to 3,275,562
_RS15680
BWI76_RS15685: BWI76_RS15685 - hypothetical protein, at 3,275,648 to 3,275,839
_RS15685
BWI76_RS15690: BWI76_RS15690 - hypothetical protein, at 3,275,790 to 3,276,065
_RS15690
BWI76_RS15695: BWI76_RS15695 - hypothetical protein, at 3,276,062 to 3,276,406
_RS15695
BWI76_RS15700: BWI76_RS15700 - hypothetical protein, at 3,276,403 to 3,276,945
_RS15700
BWI76_RS15705: BWI76_RS15705 - putative holin protein, at 3,276,942 to 3,277,253
_RS15705
Position (kb)
3276
3277 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3275.165 kb on + strand, within BWI76_RS15675 at 3275.186 kb on + strand, within BWI76_RS15675 at 3275.194 kb on - strand, within BWI76_RS15675 at 3275.194 kb on - strand, within BWI76_RS15675 at 3275.194 kb on - strand, within BWI76_RS15675 at 3275.221 kb on - strand, within BWI76_RS15675 at 3275.221 kb on - strand, within BWI76_RS15675 at 3275.278 kb on + strand at 3275.322 kb on - strand at 3275.491 kb on + strand, within BWI76_RS15680 at 3275.514 kb on - strand, within BWI76_RS15680 at 3275.533 kb on + strand, within BWI76_RS15680 at 3275.611 kb on + strand at 3275.619 kb on - strand at 3275.676 kb on - strand, within BWI76_RS15685 at 3275.808 kb on + strand at 3275.959 kb on + strand, within BWI76_RS15690 at 3276.178 kb on + strand, within BWI76_RS15695 at 3276.218 kb on - strand, within BWI76_RS15695 at 3276.253 kb on - strand at 3276.255 kb on - strand, within BWI76_RS15695 at 3276.538 kb on - strand, within BWI76_RS15700 at 3276.543 kb on - strand, within BWI76_RS15700 at 3276.606 kb on - strand, within BWI76_RS15700 at 3276.636 kb on + strand, within BWI76_RS15700 at 3276.659 kb on + strand, within BWI76_RS15700 at 3276.826 kb on - strand, within BWI76_RS15700 at 3276.971 kb on + strand at 3276.989 kb on - strand, within BWI76_RS15705 at 3276.999 kb on - strand, within BWI76_RS15705 at 3277.031 kb on - strand, within BWI76_RS15705 at 3277.062 kb on + strand, within BWI76_RS15705 at 3277.212 kb on - strand, within BWI76_RS15705 at 3277.243 kb on + strand at 3277.251 kb on - strand at 3277.300 kb on + strand at 3277.307 kb on - strand at 3277.308 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,275,165 + BWI76_RS15675 0.59 +2.4 3,275,186 + BWI76_RS15675 0.67 -0.6 3,275,194 - BWI76_RS15675 0.71 +1.3 3,275,194 - BWI76_RS15675 0.71 +1.1 3,275,194 - BWI76_RS15675 0.71 -0.8 3,275,221 - BWI76_RS15675 0.82 -0.6 3,275,221 - BWI76_RS15675 0.82 -0.8 3,275,278 + +0.2 3,275,322 - +0.2 3,275,491 + BWI76_RS15680 0.61 -0.1 3,275,514 - BWI76_RS15680 0.74 +0.1 3,275,533 + BWI76_RS15680 0.84 -0.6 3,275,611 + +1.6 3,275,619 - -0.7 3,275,676 - BWI76_RS15685 0.15 +0.4 3,275,808 + -0.5 3,275,959 + BWI76_RS15690 0.61 +2.9 3,276,178 + BWI76_RS15695 0.34 -0.4 3,276,218 - BWI76_RS15695 0.45 -0.1 3,276,253 - +1.6 3,276,255 - BWI76_RS15695 0.56 -0.8 3,276,538 - BWI76_RS15700 0.25 -0.1 3,276,543 - BWI76_RS15700 0.26 -0.5 3,276,606 - BWI76_RS15700 0.37 -1.2 3,276,636 + BWI76_RS15700 0.43 -0.4 3,276,659 + BWI76_RS15700 0.47 -0.9 3,276,826 - BWI76_RS15700 0.78 -0.5 3,276,971 + +0.3 3,276,989 - BWI76_RS15705 0.15 -0.5 3,276,999 - BWI76_RS15705 0.18 -1.0 3,277,031 - BWI76_RS15705 0.29 +2.4 3,277,062 + BWI76_RS15705 0.38 +0.9 3,277,212 - BWI76_RS15705 0.87 +0.3 3,277,243 + -0.2 3,277,251 - +0.1 3,277,300 + +0.5 3,277,307 - +0.5 3,277,308 - -1.0
Or see this region's nucleotide sequence