Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14530 and BWI76_RS14535 are separated by 86 nucleotides BWI76_RS14535 and BWI76_RS14540 overlap by 4 nucleotides
BWI76_RS14530: BWI76_RS14530 - non-ribosomal peptide synthetase, at 3,036,018 to 3,042,116
_RS14530
BWI76_RS14535: BWI76_RS14535 - polyketide synthase, at 3,042,203 to 3,051,679
_RS14535
BWI76_RS14540: BWI76_RS14540 - yersiniabactin biosynthetic protein YbtU, at 3,051,676 to 3,052,773
_RS14540
Position (kb)
3042
3043
3044
3045
3046
3047
3048
3049
3050
3051
3052 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3041.484 kb on + strand, within BWI76_RS14530 at 3041.578 kb on - strand at 3041.591 kb on + strand at 3041.697 kb on - strand at 3041.716 kb on + strand at 3041.999 kb on + strand at 3042.461 kb on + strand at 3042.688 kb on + strand at 3042.688 kb on + strand at 3042.696 kb on - strand at 3042.785 kb on - strand at 3042.836 kb on - strand at 3042.962 kb on - strand at 3043.110 kb on - strand at 3043.244 kb on + strand, within BWI76_RS14535 at 3043.280 kb on - strand, within BWI76_RS14535 at 3043.319 kb on + strand, within BWI76_RS14535 at 3043.464 kb on - strand, within BWI76_RS14535 at 3043.536 kb on - strand, within BWI76_RS14535 at 3043.586 kb on + strand, within BWI76_RS14535 at 3043.858 kb on + strand, within BWI76_RS14535 at 3043.967 kb on - strand, within BWI76_RS14535 at 3044.088 kb on + strand, within BWI76_RS14535 at 3044.284 kb on + strand, within BWI76_RS14535 at 3044.367 kb on - strand, within BWI76_RS14535 at 3044.673 kb on + strand, within BWI76_RS14535 at 3044.681 kb on - strand, within BWI76_RS14535 at 3044.737 kb on + strand, within BWI76_RS14535 at 3045.029 kb on + strand, within BWI76_RS14535 at 3045.181 kb on + strand, within BWI76_RS14535 at 3045.307 kb on - strand, within BWI76_RS14535 at 3045.578 kb on + strand, within BWI76_RS14535 at 3045.771 kb on - strand, within BWI76_RS14535 at 3046.376 kb on - strand, within BWI76_RS14535 at 3046.468 kb on + strand, within BWI76_RS14535 at 3046.528 kb on + strand, within BWI76_RS14535 at 3046.536 kb on - strand, within BWI76_RS14535 at 3047.287 kb on + strand, within BWI76_RS14535 at 3047.317 kb on + strand, within BWI76_RS14535 at 3048.087 kb on - strand, within BWI76_RS14535 at 3048.335 kb on - strand, within BWI76_RS14535 at 3049.066 kb on + strand, within BWI76_RS14535 at 3049.241 kb on + strand, within BWI76_RS14535 at 3049.531 kb on - strand, within BWI76_RS14535 at 3049.672 kb on + strand, within BWI76_RS14535 at 3050.107 kb on + strand, within BWI76_RS14535 at 3050.115 kb on - strand, within BWI76_RS14535 at 3050.509 kb on + strand, within BWI76_RS14535 at 3050.710 kb on + strand, within BWI76_RS14535 at 3050.788 kb on + strand at 3050.838 kb on + strand at 3050.875 kb on - strand at 3051.608 kb on + strand at 3051.616 kb on - strand at 3051.749 kb on + strand at 3051.848 kb on + strand, within BWI76_RS14540 at 3052.055 kb on + strand, within BWI76_RS14540 at 3052.411 kb on + strand, within BWI76_RS14540
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,041,484 + BWI76_RS14530 0.90 +2.3 3,041,578 - -0.1 3,041,591 + -0.4 3,041,697 - -0.5 3,041,716 + +0.6 3,041,999 + +1.1 3,042,461 + -0.6 3,042,688 + -2.2 3,042,688 + -0.3 3,042,696 - -0.1 3,042,785 - +2.1 3,042,836 - -1.7 3,042,962 - -1.7 3,043,110 - -0.8 3,043,244 + BWI76_RS14535 0.11 -2.1 3,043,280 - BWI76_RS14535 0.11 +0.2 3,043,319 + BWI76_RS14535 0.12 -1.9 3,043,464 - BWI76_RS14535 0.13 +0.5 3,043,536 - BWI76_RS14535 0.14 -0.2 3,043,586 + BWI76_RS14535 0.15 -1.0 3,043,858 + BWI76_RS14535 0.17 -1.1 3,043,967 - BWI76_RS14535 0.19 -0.2 3,044,088 + BWI76_RS14535 0.20 -1.4 3,044,284 + BWI76_RS14535 0.22 +0.1 3,044,367 - BWI76_RS14535 0.23 -0.2 3,044,673 + BWI76_RS14535 0.26 -0.5 3,044,681 - BWI76_RS14535 0.26 +0.2 3,044,737 + BWI76_RS14535 0.27 +0.8 3,045,029 + BWI76_RS14535 0.30 -0.6 3,045,181 + BWI76_RS14535 0.31 -0.3 3,045,307 - BWI76_RS14535 0.33 -0.4 3,045,578 + BWI76_RS14535 0.36 -0.6 3,045,771 - BWI76_RS14535 0.38 -1.3 3,046,376 - BWI76_RS14535 0.44 -0.7 3,046,468 + BWI76_RS14535 0.45 -0.1 3,046,528 + BWI76_RS14535 0.46 -0.7 3,046,536 - BWI76_RS14535 0.46 -0.4 3,047,287 + BWI76_RS14535 0.54 -0.8 3,047,317 + BWI76_RS14535 0.54 +0.8 3,048,087 - BWI76_RS14535 0.62 -0.2 3,048,335 - BWI76_RS14535 0.65 +0.4 3,049,066 + BWI76_RS14535 0.72 -0.2 3,049,241 + BWI76_RS14535 0.74 +0.2 3,049,531 - BWI76_RS14535 0.77 -1.5 3,049,672 + BWI76_RS14535 0.79 -0.7 3,050,107 + BWI76_RS14535 0.83 +0.0 3,050,115 - BWI76_RS14535 0.83 -0.6 3,050,509 + BWI76_RS14535 0.88 -0.6 3,050,710 + BWI76_RS14535 0.90 +0.9 3,050,788 + -1.5 3,050,838 + +1.6 3,050,875 - -0.4 3,051,608 + +1.3 3,051,616 - -1.3 3,051,749 + -0.8 3,051,848 + BWI76_RS14540 0.16 -0.4 3,052,055 + BWI76_RS14540 0.35 -1.0 3,052,411 + BWI76_RS14540 0.67 -1.1
Or see this region's nucleotide sequence