Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05315

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05310 and BWI76_RS05315 are separated by 15 nucleotidesBWI76_RS05315 and BWI76_RS05320 overlap by 14 nucleotidesBWI76_RS05320 and BWI76_RS05325 are separated by 196 nucleotidesBWI76_RS05325 and BWI76_RS05330 overlap by 4 nucleotides BWI76_RS05310: BWI76_RS05310 - tRNA(Ile)-lysidine synthetase, at 1,120,373 to 1,121,680 _RS05310 BWI76_RS05315: BWI76_RS05315 - Rho-binding antiterminator, at 1,121,696 to 1,121,956 _RS05315 BWI76_RS05320: BWI76_RS05320 - hypothetical protein, at 1,121,943 to 1,122,143 _RS05320 BWI76_RS05325: BWI76_RS05325 - hypothetical protein, at 1,122,340 to 1,122,885 _RS05325 BWI76_RS05330: BWI76_RS05330 - aminoacyl-tRNA hydrolase, at 1,122,882 to 1,123,295 _RS05330 Position (kb) 1121 1122Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1121.826 kb on - strand, within BWI76_RS05315at 1121.876 kb on + strand, within BWI76_RS05315at 1121.876 kb on + strand, within BWI76_RS05315at 1122.193 kb on - strandat 1122.206 kb on - strandat 1122.219 kb on + strandat 1122.463 kb on - strand, within BWI76_RS05325at 1122.595 kb on - strand, within BWI76_RS05325at 1122.743 kb on - strand, within BWI76_RS05325at 1122.754 kb on - strand, within BWI76_RS05325at 1122.762 kb on + strand, within BWI76_RS05325at 1122.813 kb on - strand, within BWI76_RS05325at 1122.813 kb on - strand, within BWI76_RS05325

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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1,121,826 - BWI76_RS05315 0.50 -0.4
1,121,876 + BWI76_RS05315 0.69 -1.1
1,121,876 + BWI76_RS05315 0.69 -0.1
1,122,193 - -1.9
1,122,206 - +0.1
1,122,219 + +0.3
1,122,463 - BWI76_RS05325 0.23 +3.5
1,122,595 - BWI76_RS05325 0.47 +0.3
1,122,743 - BWI76_RS05325 0.74 -0.2
1,122,754 - BWI76_RS05325 0.76 -0.2
1,122,762 + BWI76_RS05325 0.77 +0.3
1,122,813 - BWI76_RS05325 0.87 +1.7
1,122,813 - BWI76_RS05325 0.87 +0.0

Or see this region's nucleotide sequence