Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04205 and BWI76_RS04210 are separated by 209 nucleotides BWI76_RS04210 and BWI76_RS04215 are separated by 12 nucleotides BWI76_RS04215 and BWI76_RS04220 overlap by 1 nucleotides
BWI76_RS04205: BWI76_RS04205 - right oriC-binding transcriptional activator, at 864,977 to 865,846
_RS04205
BWI76_RS04210: BWI76_RS04210 - hypothetical protein, at 866,056 to 866,529
_RS04210
BWI76_RS04215: BWI76_RS04215 - DNA-binding response regulator, at 866,542 to 867,231
_RS04215
BWI76_RS04220: BWI76_RS04220 - two-component sensor histidine kinase, at 867,231 to 868,655
_RS04220
Position (kb) 866
867
868 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 865.551 kb on - strand, within BWI76_RS04205 at 865.653 kb on - strand, within BWI76_RS04205 at 865.759 kb on - strand, within BWI76_RS04205 at 865.767 kb on + strand at 865.800 kb on + strand at 865.809 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.811 kb on + strand at 865.819 kb on - strand at 865.819 kb on - strand at 865.819 kb on - strand at 865.819 kb on - strand at 865.819 kb on - strand at 865.859 kb on + strand at 865.859 kb on + strand at 865.859 kb on + strand at 865.866 kb on + strand at 866.247 kb on + strand, within BWI76_RS04210 at 866.258 kb on + strand, within BWI76_RS04210 at 866.279 kb on + strand, within BWI76_RS04210 at 866.279 kb on + strand, within BWI76_RS04210 at 866.287 kb on - strand, within BWI76_RS04210 at 866.551 kb on + strand at 866.598 kb on + strand at 866.606 kb on - strand at 866.606 kb on - strand at 866.734 kb on - strand, within BWI76_RS04215 at 866.786 kb on - strand, within BWI76_RS04215 at 866.786 kb on - strand, within BWI76_RS04215 at 866.803 kb on - strand, within BWI76_RS04215 at 867.088 kb on + strand, within BWI76_RS04215 at 867.103 kb on + strand, within BWI76_RS04215 at 867.132 kb on - strand, within BWI76_RS04215 at 867.215 kb on + strand at 867.215 kb on + strand at 867.356 kb on + strand at 867.533 kb on + strand, within BWI76_RS04220 at 867.541 kb on - strand, within BWI76_RS04220 at 867.541 kb on - strand, within BWI76_RS04220 at 867.707 kb on + strand, within BWI76_RS04220 at 867.714 kb on + strand, within BWI76_RS04220 at 867.775 kb on + strand, within BWI76_RS04220 at 867.928 kb on + strand, within BWI76_RS04220 at 867.928 kb on + strand, within BWI76_RS04220 at 867.936 kb on - strand, within BWI76_RS04220 at 867.936 kb on - strand, within BWI76_RS04220 at 867.936 kb on - strand, within BWI76_RS04220 at 867.948 kb on + strand, within BWI76_RS04220 at 867.970 kb on + strand, within BWI76_RS04220 at 867.970 kb on + strand, within BWI76_RS04220 at 868.189 kb on + strand, within BWI76_RS04220
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 865,551 - BWI76_RS04205 0.66 -0.4 865,653 - BWI76_RS04205 0.78 +0.9 865,759 - BWI76_RS04205 0.90 +0.5 865,767 + +1.1 865,800 + +0.7 865,809 + +0.4 865,811 + +1.7 865,811 + +0.1 865,811 + -1.2 865,811 + -1.6 865,811 + +1.3 865,811 + +1.0 865,811 + +0.7 865,819 - +0.7 865,819 - +1.0 865,819 - +0.6 865,819 - +0.3 865,819 - +0.4 865,859 + +1.2 865,859 + +1.6 865,859 + -0.7 865,866 + -0.3 866,247 + BWI76_RS04210 0.40 +1.6 866,258 + BWI76_RS04210 0.43 +0.2 866,279 + BWI76_RS04210 0.47 -0.3 866,279 + BWI76_RS04210 0.47 -1.6 866,287 - BWI76_RS04210 0.49 -1.1 866,551 + +0.2 866,598 + -0.5 866,606 - -0.5 866,606 - -1.9 866,734 - BWI76_RS04215 0.28 +0.6 866,786 - BWI76_RS04215 0.35 +4.1 866,786 - BWI76_RS04215 0.35 +0.6 866,803 - BWI76_RS04215 0.38 -0.1 867,088 + BWI76_RS04215 0.79 -1.0 867,103 + BWI76_RS04215 0.81 +0.1 867,132 - BWI76_RS04215 0.86 +0.8 867,215 + -0.4 867,215 + -0.9 867,356 + -0.8 867,533 + BWI76_RS04220 0.21 +1.1 867,541 - BWI76_RS04220 0.22 +0.6 867,541 - BWI76_RS04220 0.22 -0.2 867,707 + BWI76_RS04220 0.33 -1.6 867,714 + BWI76_RS04220 0.34 -0.4 867,775 + BWI76_RS04220 0.38 -1.9 867,928 + BWI76_RS04220 0.49 -0.5 867,928 + BWI76_RS04220 0.49 -0.6 867,936 - BWI76_RS04220 0.49 +1.3 867,936 - BWI76_RS04220 0.49 -0.6 867,936 - BWI76_RS04220 0.49 -0.4 867,948 + BWI76_RS04220 0.50 +0.5 867,970 + BWI76_RS04220 0.52 +3.4 867,970 + BWI76_RS04220 0.52 -1.3 868,189 + BWI76_RS04220 0.67 +0.5
Or see this region's nucleotide sequence