Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27165 and BWI76_RS27170 overlap by 4 nucleotides BWI76_RS27170 and BWI76_RS27175 overlap by 12 nucleotides BWI76_RS27175 and BWI76_RS27180 are separated by 274 nucleotides
BWI76_RS27165: BWI76_RS27165 - mannitol-1-phosphate 5-dehydrogenase, at 5,640,050 to 5,641,198
_RS27165
BWI76_RS27170: BWI76_RS27170 - MltR family transcriptional regulator, at 5,641,195 to 5,641,791
_RS27170
BWI76_RS27175: BWI76_RS27175 - hypothetical protein, at 5,641,780 to 5,642,004
_RS27175
BWI76_RS27180: BWI76_RS27180 - hypothetical protein, at 5,642,279 to 5,642,641
_RS27180
Position (kb) 5641
5642 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5640.225 kb on - strand, within BWI76_RS27165 at 5640.335 kb on + strand, within BWI76_RS27165 at 5640.444 kb on - strand, within BWI76_RS27165 at 5640.523 kb on - strand, within BWI76_RS27165 at 5640.613 kb on + strand, within BWI76_RS27165 at 5640.621 kb on - strand, within BWI76_RS27165 at 5640.621 kb on - strand, within BWI76_RS27165 at 5640.621 kb on - strand, within BWI76_RS27165 at 5640.686 kb on + strand, within BWI76_RS27165 at 5640.990 kb on - strand, within BWI76_RS27165 at 5640.996 kb on + strand, within BWI76_RS27165 at 5641.215 kb on + strand at 5641.434 kb on + strand, within BWI76_RS27170 at 5641.487 kb on + strand, within BWI76_RS27170 at 5641.495 kb on - strand, within BWI76_RS27170 at 5641.538 kb on - strand, within BWI76_RS27170 at 5641.549 kb on + strand, within BWI76_RS27170 at 5641.567 kb on + strand, within BWI76_RS27170 at 5641.570 kb on + strand, within BWI76_RS27170 at 5641.664 kb on + strand, within BWI76_RS27170 at 5641.705 kb on + strand, within BWI76_RS27170 at 5641.736 kb on - strand at 5641.736 kb on - strand at 5641.798 kb on - strand at 5641.839 kb on + strand, within BWI76_RS27175 at 5641.840 kb on - strand, within BWI76_RS27175 at 5641.889 kb on + strand, within BWI76_RS27175 at 5641.941 kb on - strand, within BWI76_RS27175 at 5642.008 kb on + strand at 5642.008 kb on + strand at 5642.011 kb on + strand at 5642.011 kb on + strand at 5642.013 kb on + strand at 5642.016 kb on - strand at 5642.016 kb on - strand at 5642.018 kb on + strand at 5642.019 kb on - strand at 5642.019 kb on - strand at 5642.019 kb on - strand at 5642.023 kb on + strand at 5642.031 kb on - strand at 5642.039 kb on + strand at 5642.085 kb on + strand at 5642.159 kb on - strand at 5642.218 kb on + strand at 5642.218 kb on + strand at 5642.226 kb on - strand at 5642.228 kb on - strand at 5642.230 kb on + strand at 5642.231 kb on - strand at 5642.232 kb on + strand at 5642.236 kb on + strand at 5642.238 kb on - strand at 5642.267 kb on + strand at 5642.323 kb on + strand, within BWI76_RS27180 at 5642.323 kb on + strand, within BWI76_RS27180 at 5642.323 kb on + strand, within BWI76_RS27180 at 5642.324 kb on - strand, within BWI76_RS27180 at 5642.348 kb on + strand, within BWI76_RS27180 at 5642.356 kb on - strand, within BWI76_RS27180 at 5642.362 kb on + strand, within BWI76_RS27180 at 5642.370 kb on - strand, within BWI76_RS27180 at 5642.440 kb on + strand, within BWI76_RS27180 at 5642.448 kb on + strand, within BWI76_RS27180 at 5642.449 kb on - strand, within BWI76_RS27180 at 5642.449 kb on - strand, within BWI76_RS27180 at 5642.458 kb on + strand, within BWI76_RS27180 at 5642.691 kb on - strand at 5642.767 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 5,640,225 - BWI76_RS27165 0.15 +0.4 5,640,335 + BWI76_RS27165 0.25 -1.1 5,640,444 - BWI76_RS27165 0.34 -1.0 5,640,523 - BWI76_RS27165 0.41 -1.9 5,640,613 + BWI76_RS27165 0.49 +0.4 5,640,621 - BWI76_RS27165 0.50 -1.5 5,640,621 - BWI76_RS27165 0.50 -1.9 5,640,621 - BWI76_RS27165 0.50 -1.4 5,640,686 + BWI76_RS27165 0.55 -0.3 5,640,990 - BWI76_RS27165 0.82 +1.6 5,640,996 + BWI76_RS27165 0.82 -1.1 5,641,215 + -0.8 5,641,434 + BWI76_RS27170 0.40 -1.7 5,641,487 + BWI76_RS27170 0.49 -0.5 5,641,495 - BWI76_RS27170 0.50 -0.9 5,641,538 - BWI76_RS27170 0.57 -0.5 5,641,549 + BWI76_RS27170 0.59 +0.0 5,641,567 + BWI76_RS27170 0.62 -0.9 5,641,570 + BWI76_RS27170 0.63 -2.0 5,641,664 + BWI76_RS27170 0.79 -0.1 5,641,705 + BWI76_RS27170 0.85 -2.3 5,641,736 - -2.0 5,641,736 - -1.4 5,641,798 - -2.8 5,641,839 + BWI76_RS27175 0.26 -1.8 5,641,840 - BWI76_RS27175 0.27 +2.6 5,641,889 + BWI76_RS27175 0.48 +0.7 5,641,941 - BWI76_RS27175 0.72 +0.4 5,642,008 + -0.2 5,642,008 + -0.1 5,642,011 + -1.0 5,642,011 + +0.9 5,642,013 + +0.1 5,642,016 - +1.7 5,642,016 - -0.3 5,642,018 + +1.3 5,642,019 - +0.0 5,642,019 - -1.2 5,642,019 - -0.8 5,642,023 + -0.8 5,642,031 - -0.4 5,642,039 + +0.3 5,642,085 + +0.2 5,642,159 - +2.0 5,642,218 + -1.2 5,642,218 + -0.4 5,642,226 - -1.1 5,642,228 - -1.9 5,642,230 + +0.3 5,642,231 - +0.9 5,642,232 + -1.4 5,642,236 + -1.2 5,642,238 - -0.7 5,642,267 + +1.7 5,642,323 + BWI76_RS27180 0.12 -0.4 5,642,323 + BWI76_RS27180 0.12 -0.2 5,642,323 + BWI76_RS27180 0.12 -0.1 5,642,324 - BWI76_RS27180 0.12 -0.5 5,642,348 + BWI76_RS27180 0.19 +0.6 5,642,356 - BWI76_RS27180 0.21 -0.2 5,642,362 + BWI76_RS27180 0.23 +0.8 5,642,370 - BWI76_RS27180 0.25 +0.3 5,642,440 + BWI76_RS27180 0.44 +0.4 5,642,448 + BWI76_RS27180 0.47 +0.5 5,642,449 - BWI76_RS27180 0.47 +2.2 5,642,449 - BWI76_RS27180 0.47 +0.2 5,642,458 + BWI76_RS27180 0.49 +1.0 5,642,691 - -0.9 5,642,767 + -2.1
Or see this region's nucleotide sequence