Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04465 and BWI76_RS04470 are separated by 18 nucleotides BWI76_RS04470 and BWI76_RS04475 are separated by 138 nucleotides
BWI76_RS04465: BWI76_RS04465 - carbamoyl-phosphate synthase small subunit, at 922,546 to 923,694
_RS04465
BWI76_RS04470: BWI76_RS04470 - carbamoyl phosphate synthase large subunit, at 923,713 to 926,937
_RS04470
BWI76_RS04475: BWI76_RS04475 - ROK family protein, at 927,076 to 927,960
_RS04475
Position (kb) 923
924
925
926
927 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 922.828 kb on + strand, within BWI76_RS04465 at 922.836 kb on + strand, within BWI76_RS04465 at 922.836 kb on - strand, within BWI76_RS04465 at 922.897 kb on - strand, within BWI76_RS04465 at 923.052 kb on - strand, within BWI76_RS04465 at 923.206 kb on + strand, within BWI76_RS04465 at 923.623 kb on - strand at 923.628 kb on - strand at 923.794 kb on - strand at 923.808 kb on + strand at 923.816 kb on - strand at 923.855 kb on + strand at 923.862 kb on + strand at 923.879 kb on - strand at 923.995 kb on + strand at 923.995 kb on + strand at 924.003 kb on - strand at 924.256 kb on + strand, within BWI76_RS04470 at 924.264 kb on - strand at 924.575 kb on - strand, within BWI76_RS04470 at 924.717 kb on + strand, within BWI76_RS04470 at 924.748 kb on - strand, within BWI76_RS04470 at 924.751 kb on - strand, within BWI76_RS04470 at 924.760 kb on - strand, within BWI76_RS04470 at 924.914 kb on + strand, within BWI76_RS04470 at 925.097 kb on + strand, within BWI76_RS04470 at 925.338 kb on + strand, within BWI76_RS04470 at 925.346 kb on - strand, within BWI76_RS04470 at 925.547 kb on + strand, within BWI76_RS04470 at 925.706 kb on + strand, within BWI76_RS04470 at 925.931 kb on - strand, within BWI76_RS04470 at 926.164 kb on - strand, within BWI76_RS04470 at 926.332 kb on - strand, within BWI76_RS04470 at 926.473 kb on + strand, within BWI76_RS04470 at 926.481 kb on - strand, within BWI76_RS04470 at 926.610 kb on + strand, within BWI76_RS04470 at 926.817 kb on + strand at 926.817 kb on + strand at 926.903 kb on - strand at 927.074 kb on - strand at 927.433 kb on + strand, within BWI76_RS04475 at 927.433 kb on + strand, within BWI76_RS04475 at 927.441 kb on - strand, within BWI76_RS04475 at 927.613 kb on - strand, within BWI76_RS04475 at 927.747 kb on + strand, within BWI76_RS04475 at 927.752 kb on + strand, within BWI76_RS04475 at 927.815 kb on + strand, within BWI76_RS04475 at 927.823 kb on - strand, within BWI76_RS04475 at 927.907 kb on + strand at 927.910 kb on - strand at 927.915 kb on - strand at 927.922 kb on + strand at 927.930 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 922,828 + BWI76_RS04465 0.25 -2.7 922,836 + BWI76_RS04465 0.25 -2.2 922,836 - BWI76_RS04465 0.25 -0.6 922,897 - BWI76_RS04465 0.31 +0.6 923,052 - BWI76_RS04465 0.44 -2.9 923,206 + BWI76_RS04465 0.57 +1.8 923,623 - -1.1 923,628 - -1.2 923,794 - -1.6 923,808 + -1.1 923,816 - -0.2 923,855 + -1.1 923,862 + -0.8 923,879 - +0.1 923,995 + -1.6 923,995 + -0.4 924,003 - -1.8 924,256 + BWI76_RS04470 0.17 -1.4 924,264 - +1.6 924,575 - BWI76_RS04470 0.27 -0.4 924,717 + BWI76_RS04470 0.31 -0.4 924,748 - BWI76_RS04470 0.32 -1.6 924,751 - BWI76_RS04470 0.32 -0.0 924,760 - BWI76_RS04470 0.32 -1.4 924,914 + BWI76_RS04470 0.37 -3.4 925,097 + BWI76_RS04470 0.43 -1.3 925,338 + BWI76_RS04470 0.50 -0.8 925,346 - BWI76_RS04470 0.51 -1.8 925,547 + BWI76_RS04470 0.57 -2.8 925,706 + BWI76_RS04470 0.62 -0.6 925,931 - BWI76_RS04470 0.69 -1.1 926,164 - BWI76_RS04470 0.76 -0.8 926,332 - BWI76_RS04470 0.81 -1.2 926,473 + BWI76_RS04470 0.86 -0.4 926,481 - BWI76_RS04470 0.86 -2.6 926,610 + BWI76_RS04470 0.90 -0.6 926,817 + -0.5 926,817 + -0.4 926,903 - -0.4 927,074 - -0.0 927,433 + BWI76_RS04475 0.40 -0.8 927,433 + BWI76_RS04475 0.40 -1.8 927,441 - BWI76_RS04475 0.41 +1.5 927,613 - BWI76_RS04475 0.61 +0.2 927,747 + BWI76_RS04475 0.76 -0.6 927,752 + BWI76_RS04475 0.76 +0.0 927,815 + BWI76_RS04475 0.84 -0.2 927,823 - BWI76_RS04475 0.84 -0.3 927,907 + +0.2 927,910 - -0.5 927,915 - -0.2 927,922 + +0.5 927,930 - -0.8
Or see this region's nucleotide sequence