Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05730 and BWI76_RS05735 are separated by 99 nucleotides BWI76_RS05735 and BWI76_RS05740 overlap by 14 nucleotides BWI76_RS05740 and BWI76_RS05745 are separated by 143 nucleotides BWI76_RS05745 and BWI76_RS05750 are separated by 72 nucleotides
BWI76_RS05730: BWI76_RS05730 - hypothetical protein, at 1,206,461 to 1,207,207
_RS05730
BWI76_RS05735: BWI76_RS05735 - transcriptional regulator, at 1,207,307 to 1,207,675
_RS05735
BWI76_RS05740: BWI76_RS05740 - hypothetical protein, at 1,207,662 to 1,207,898
_RS05740
BWI76_RS05745: BWI76_RS05745 - hypothetical protein, at 1,208,042 to 1,209,280
_RS05745
BWI76_RS05750: BWI76_RS05750 - thiamine biosynthesis protein ThiJ, at 1,209,353 to 1,210,048
_RS05750
Position (kb)
1208
1209
1210 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1207.050 kb on + strand, within BWI76_RS05730 at 1207.050 kb on + strand, within BWI76_RS05730 at 1207.060 kb on - strand, within BWI76_RS05730 at 1207.101 kb on - strand, within BWI76_RS05730 at 1207.102 kb on + strand, within BWI76_RS05730 at 1207.110 kb on - strand, within BWI76_RS05730 at 1207.235 kb on + strand at 1207.241 kb on + strand at 1207.248 kb on + strand at 1207.256 kb on - strand at 1207.373 kb on + strand, within BWI76_RS05735 at 1207.449 kb on + strand, within BWI76_RS05735 at 1207.503 kb on + strand, within BWI76_RS05735 at 1207.503 kb on + strand, within BWI76_RS05735 at 1207.511 kb on - strand, within BWI76_RS05735 at 1207.551 kb on - strand, within BWI76_RS05735 at 1207.556 kb on + strand, within BWI76_RS05735 at 1207.566 kb on + strand, within BWI76_RS05735 at 1207.586 kb on + strand, within BWI76_RS05735 at 1207.588 kb on + strand, within BWI76_RS05735 at 1207.593 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.601 kb on - strand, within BWI76_RS05735 at 1207.603 kb on + strand, within BWI76_RS05735 at 1207.606 kb on - strand, within BWI76_RS05735 at 1207.618 kb on - strand, within BWI76_RS05735 at 1207.654 kb on + strand at 1207.659 kb on + strand at 1207.659 kb on + strand at 1207.667 kb on - strand at 1207.667 kb on - strand at 1207.667 kb on - strand at 1207.678 kb on - strand at 1207.701 kb on - strand, within BWI76_RS05740 at 1207.744 kb on + strand, within BWI76_RS05740 at 1207.768 kb on + strand, within BWI76_RS05740 at 1207.775 kb on + strand, within BWI76_RS05740 at 1207.775 kb on + strand, within BWI76_RS05740 at 1207.811 kb on - strand, within BWI76_RS05740 at 1207.839 kb on - strand, within BWI76_RS05740 at 1207.839 kb on - strand, within BWI76_RS05740 at 1208.008 kb on + strand at 1209.263 kb on + strand at 1209.461 kb on + strand, within BWI76_RS05750 at 1209.473 kb on + strand, within BWI76_RS05750 at 1209.473 kb on + strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.610 kb on + strand, within BWI76_RS05750 at 1209.616 kb on - strand, within BWI76_RS05750 at 1209.639 kb on + strand, within BWI76_RS05750 at 1209.703 kb on + strand, within BWI76_RS05750 at 1209.736 kb on + strand, within BWI76_RS05750 at 1209.817 kb on + strand, within BWI76_RS05750 at 1209.820 kb on - strand, within BWI76_RS05750 at 1209.825 kb on - strand, within BWI76_RS05750 at 1209.870 kb on - strand, within BWI76_RS05750 at 1209.921 kb on - strand, within BWI76_RS05750 at 1209.967 kb on - strand, within BWI76_RS05750 at 1209.994 kb on + strand at 1209.994 kb on + strand at 1210.012 kb on + strand at 1210.012 kb on + strand at 1210.012 kb on + strand at 1210.020 kb on - strand at 1210.105 kb on + strand at 1210.107 kb on + strand at 1210.113 kb on - strand at 1210.116 kb on + strand at 1210.131 kb on + strand at 1210.139 kb on - strand at 1210.175 kb on - strand at 1210.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 1,207,050 + BWI76_RS05730 0.79 -0.2 1,207,050 + BWI76_RS05730 0.79 -0.1 1,207,060 - BWI76_RS05730 0.80 +0.0 1,207,101 - BWI76_RS05730 0.86 +0.6 1,207,102 + BWI76_RS05730 0.86 -0.4 1,207,110 - BWI76_RS05730 0.87 +0.4 1,207,235 + -1.4 1,207,241 + +0.5 1,207,248 + -1.4 1,207,256 - -0.0 1,207,373 + BWI76_RS05735 0.18 -0.0 1,207,449 + BWI76_RS05735 0.38 -0.2 1,207,503 + BWI76_RS05735 0.53 -0.7 1,207,503 + BWI76_RS05735 0.53 -1.7 1,207,511 - BWI76_RS05735 0.55 +0.7 1,207,551 - BWI76_RS05735 0.66 -0.0 1,207,556 + BWI76_RS05735 0.67 -0.3 1,207,566 + BWI76_RS05735 0.70 +0.6 1,207,586 + BWI76_RS05735 0.76 -1.1 1,207,588 + BWI76_RS05735 0.76 -3.5 1,207,593 + BWI76_RS05735 0.78 +0.1 1,207,598 + BWI76_RS05735 0.79 -0.5 1,207,598 + BWI76_RS05735 0.79 +0.6 1,207,598 + BWI76_RS05735 0.79 +0.0 1,207,601 - BWI76_RS05735 0.80 -0.3 1,207,603 + BWI76_RS05735 0.80 -0.6 1,207,606 - BWI76_RS05735 0.81 -0.7 1,207,618 - BWI76_RS05735 0.84 +0.1 1,207,654 + -0.4 1,207,659 + -0.0 1,207,659 + -0.4 1,207,667 - +1.5 1,207,667 - +0.0 1,207,667 - -0.1 1,207,678 - +0.5 1,207,701 - BWI76_RS05740 0.16 +0.4 1,207,744 + BWI76_RS05740 0.35 +1.0 1,207,768 + BWI76_RS05740 0.45 +0.0 1,207,775 + BWI76_RS05740 0.48 +0.0 1,207,775 + BWI76_RS05740 0.48 +0.6 1,207,811 - BWI76_RS05740 0.63 -0.6 1,207,839 - BWI76_RS05740 0.75 -0.3 1,207,839 - BWI76_RS05740 0.75 +0.7 1,208,008 + -2.1 1,209,263 + -1.4 1,209,461 + BWI76_RS05750 0.16 -1.3 1,209,473 + BWI76_RS05750 0.17 +1.2 1,209,473 + BWI76_RS05750 0.17 +1.0 1,209,481 - BWI76_RS05750 0.18 +0.5 1,209,481 - BWI76_RS05750 0.18 -1.3 1,209,481 - BWI76_RS05750 0.18 -1.9 1,209,610 + BWI76_RS05750 0.37 -0.3 1,209,616 - BWI76_RS05750 0.38 +0.2 1,209,639 + BWI76_RS05750 0.41 +0.6 1,209,703 + BWI76_RS05750 0.50 -0.2 1,209,736 + BWI76_RS05750 0.55 -0.4 1,209,817 + BWI76_RS05750 0.67 -0.1 1,209,820 - BWI76_RS05750 0.67 -0.3 1,209,825 - BWI76_RS05750 0.68 -1.2 1,209,870 - BWI76_RS05750 0.74 +1.0 1,209,921 - BWI76_RS05750 0.82 +0.1 1,209,967 - BWI76_RS05750 0.88 -0.9 1,209,994 + -0.4 1,209,994 + +0.4 1,210,012 + -0.1 1,210,012 + +0.7 1,210,012 + +0.5 1,210,020 - -1.4 1,210,105 + +2.2 1,210,107 + -1.5 1,210,113 - +0.6 1,210,116 + -0.5 1,210,131 + +0.9 1,210,139 - +1.6 1,210,175 - -2.3 1,210,280 - -0.7
Or see this region's nucleotide sequence