Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11040 and BWI76_RS11045 are separated by 307 nucleotides BWI76_RS11045 and BWI76_RS11050 are separated by 189 nucleotides BWI76_RS11050 and BWI76_RS11055 are separated by 12 nucleotides
BWI76_RS11040: BWI76_RS11040 - ribonuclease E, at 2,328,509 to 2,331,757
_RS11040
BWI76_RS11045: BWI76_RS11045 - hypothetical protein, at 2,332,065 to 2,332,223
_RS11045
BWI76_RS11050: BWI76_RS11050 - 23S rRNA pseudouridine(955/2504/2580) synthase, at 2,332,413 to 2,333,408
_RS11050
BWI76_RS11055: BWI76_RS11055 - hydrolase, at 2,333,421 to 2,334,566
_RS11055
Position (kb)
2332
2333
2334 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2332.246 kb on - strand at 2332.334 kb on - strand at 2332.375 kb on + strand at 2332.420 kb on + strand at 2332.541 kb on + strand, within BWI76_RS11050 at 2332.547 kb on - strand, within BWI76_RS11050 at 2332.547 kb on - strand, within BWI76_RS11050 at 2332.549 kb on - strand, within BWI76_RS11050 at 2332.549 kb on - strand, within BWI76_RS11050 at 2332.585 kb on + strand, within BWI76_RS11050 at 2332.699 kb on + strand, within BWI76_RS11050 at 2332.806 kb on + strand, within BWI76_RS11050 at 2332.813 kb on + strand, within BWI76_RS11050 at 2332.821 kb on - strand, within BWI76_RS11050 at 2332.989 kb on - strand, within BWI76_RS11050 at 2333.186 kb on - strand, within BWI76_RS11050 at 2333.211 kb on - strand, within BWI76_RS11050 at 2333.570 kb on + strand, within BWI76_RS11055 at 2333.838 kb on + strand, within BWI76_RS11055 at 2334.197 kb on + strand, within BWI76_RS11055 at 2334.286 kb on - strand, within BWI76_RS11055 at 2334.373 kb on - strand, within BWI76_RS11055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 2,332,246 - -0.2 2,332,334 - -0.1 2,332,375 + -0.0 2,332,420 + +1.9 2,332,541 + BWI76_RS11050 0.13 -1.6 2,332,547 - BWI76_RS11050 0.13 +0.2 2,332,547 - BWI76_RS11050 0.13 -0.9 2,332,549 - BWI76_RS11050 0.14 +0.1 2,332,549 - BWI76_RS11050 0.14 -1.6 2,332,585 + BWI76_RS11050 0.17 -1.0 2,332,699 + BWI76_RS11050 0.29 +0.8 2,332,806 + BWI76_RS11050 0.39 +0.4 2,332,813 + BWI76_RS11050 0.40 -2.0 2,332,821 - BWI76_RS11050 0.41 -0.8 2,332,989 - BWI76_RS11050 0.58 -1.4 2,333,186 - BWI76_RS11050 0.78 -1.2 2,333,211 - BWI76_RS11050 0.80 +0.4 2,333,570 + BWI76_RS11055 0.13 +0.2 2,333,838 + BWI76_RS11055 0.36 -0.4 2,334,197 + BWI76_RS11055 0.68 +0.3 2,334,286 - BWI76_RS11055 0.75 +1.1 2,334,373 - BWI76_RS11055 0.83 -1.1
Or see this region's nucleotide sequence