Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10385

Experiment: R2A with Vogels_fungal_media 0.2X

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10375 and BWI76_RS10380 are separated by 13 nucleotidesBWI76_RS10380 and BWI76_RS10385 are separated by 142 nucleotidesBWI76_RS10385 and BWI76_RS10390 are separated by 4 nucleotides BWI76_RS10375: BWI76_RS10375 - polar amino acid ABC transporter inner membrane subunit, at 2,196,857 to 2,197,522 _RS10375 BWI76_RS10380: BWI76_RS10380 - polar amino acid ABC transporter inner membrane subunit, at 2,197,536 to 2,198,207 _RS10380 BWI76_RS10385: BWI76_RS10385 - MmgE/PrpD family protein, at 2,198,350 to 2,199,759 _RS10385 BWI76_RS10390: BWI76_RS10390 - amino acid ABC transporter, at 2,199,764 to 2,200,588 _RS10390 Position (kb) 2198 2199 2200Strain fitness (log2 ratio) -2 -1 0 1 2at 2197.551 kb on - strandat 2197.617 kb on - strand, within BWI76_RS10380at 2197.683 kb on - strand, within BWI76_RS10380at 2198.137 kb on + strand, within BWI76_RS10380at 2198.140 kb on + strandat 2198.209 kb on + strandat 2198.262 kb on + strandat 2198.433 kb on + strandat 2198.606 kb on - strand, within BWI76_RS10385at 2198.832 kb on - strand, within BWI76_RS10385at 2199.436 kb on - strand, within BWI76_RS10385at 2199.577 kb on + strand, within BWI76_RS10385at 2199.805 kb on + strandat 2199.813 kb on - strandat 2199.820 kb on + strandat 2199.820 kb on + strandat 2199.820 kb on + strandat 2199.820 kb on + strandat 2199.820 kb on + strandat 2199.820 kb on + strandat 2199.828 kb on - strandat 2199.828 kb on - strandat 2199.828 kb on - strandat 2199.828 kb on - strandat 2200.149 kb on - strand, within BWI76_RS10390at 2200.233 kb on + strand, within BWI76_RS10390at 2200.235 kb on - strand, within BWI76_RS10390at 2200.299 kb on + strand, within BWI76_RS10390at 2200.307 kb on - strand, within BWI76_RS10390at 2200.307 kb on - strand, within BWI76_RS10390at 2200.307 kb on - strand, within BWI76_RS10390at 2200.337 kb on + strand, within BWI76_RS10390at 2200.341 kb on - strand, within BWI76_RS10390at 2200.371 kb on - strand, within BWI76_RS10390at 2200.387 kb on + strand, within BWI76_RS10390at 2200.398 kb on + strand, within BWI76_RS10390at 2200.399 kb on + strandat 2200.407 kb on - strand, within BWI76_RS10390at 2200.408 kb on - strand, within BWI76_RS10390at 2200.408 kb on - strand, within BWI76_RS10390at 2200.413 kb on - strand, within BWI76_RS10390at 2200.413 kb on - strand, within BWI76_RS10390at 2200.438 kb on - strand, within BWI76_RS10390at 2200.452 kb on + strand, within BWI76_RS10390at 2200.460 kb on - strand, within BWI76_RS10390at 2200.460 kb on - strand, within BWI76_RS10390at 2200.460 kb on - strand, within BWI76_RS10390at 2200.460 kb on - strand, within BWI76_RS10390at 2200.460 kb on - strand, within BWI76_RS10390at 2200.460 kb on - strand, within BWI76_RS10390at 2200.460 kb on - strand, within BWI76_RS10390at 2200.465 kb on - strand, within BWI76_RS10390at 2200.465 kb on - strand, within BWI76_RS10390at 2200.465 kb on - strandat 2200.467 kb on + strand, within BWI76_RS10390at 2200.470 kb on - strand, within BWI76_RS10390at 2200.470 kb on - strand, within BWI76_RS10390at 2200.475 kb on - strand, within BWI76_RS10390at 2200.475 kb on - strand, within BWI76_RS10390at 2200.514 kb on + strandat 2200.522 kb on - strandat 2200.548 kb on + strandat 2200.598 kb on - strandat 2200.650 kb on - strandat 2200.650 kb on - strandat 2200.694 kb on - strandat 2200.694 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X
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2,197,551 - +0.3
2,197,617 - BWI76_RS10380 0.12 -0.4
2,197,683 - BWI76_RS10380 0.22 -0.2
2,198,137 + BWI76_RS10380 0.89 +0.0
2,198,140 + +0.4
2,198,209 + -0.3
2,198,262 + +0.4
2,198,433 + -0.2
2,198,606 - BWI76_RS10385 0.18 -0.2
2,198,832 - BWI76_RS10385 0.34 -0.4
2,199,436 - BWI76_RS10385 0.77 -2.3
2,199,577 + BWI76_RS10385 0.87 -1.3
2,199,805 + +0.2
2,199,813 - +0.2
2,199,820 + +0.8
2,199,820 + +1.1
2,199,820 + -0.4
2,199,820 + +0.5
2,199,820 + -0.3
2,199,820 + -2.7
2,199,828 - +0.2
2,199,828 - -0.1
2,199,828 - +0.0
2,199,828 - -0.2
2,200,149 - BWI76_RS10390 0.47 -0.1
2,200,233 + BWI76_RS10390 0.57 -0.5
2,200,235 - BWI76_RS10390 0.57 -1.0
2,200,299 + BWI76_RS10390 0.65 +0.4
2,200,307 - BWI76_RS10390 0.66 +0.3
2,200,307 - BWI76_RS10390 0.66 +0.1
2,200,307 - BWI76_RS10390 0.66 -0.0
2,200,337 + BWI76_RS10390 0.69 -0.8
2,200,341 - BWI76_RS10390 0.70 +0.3
2,200,371 - BWI76_RS10390 0.74 -2.7
2,200,387 + BWI76_RS10390 0.76 -0.9
2,200,398 + BWI76_RS10390 0.77 -0.1
2,200,399 + -0.1
2,200,407 - BWI76_RS10390 0.78 +0.8
2,200,408 - BWI76_RS10390 0.78 +1.1
2,200,408 - BWI76_RS10390 0.78 -0.7
2,200,413 - BWI76_RS10390 0.79 -0.1
2,200,413 - BWI76_RS10390 0.79 +0.1
2,200,438 - BWI76_RS10390 0.82 -0.3
2,200,452 + BWI76_RS10390 0.83 +0.7
2,200,460 - BWI76_RS10390 0.84 -0.3
2,200,460 - BWI76_RS10390 0.84 -0.1
2,200,460 - BWI76_RS10390 0.84 -0.1
2,200,460 - BWI76_RS10390 0.84 +0.0
2,200,460 - BWI76_RS10390 0.84 +0.7
2,200,460 - BWI76_RS10390 0.84 -0.4
2,200,460 - BWI76_RS10390 0.84 -0.7
2,200,465 - BWI76_RS10390 0.85 +0.1
2,200,465 - BWI76_RS10390 0.85 -1.0
2,200,465 - -0.7
2,200,467 + BWI76_RS10390 0.85 -2.4
2,200,470 - BWI76_RS10390 0.86 -0.9
2,200,470 - BWI76_RS10390 0.86 -0.9
2,200,475 - BWI76_RS10390 0.86 +0.1
2,200,475 - BWI76_RS10390 0.86 -0.4
2,200,514 + +1.1
2,200,522 - +1.4
2,200,548 + +2.1
2,200,598 - +0.3
2,200,650 - -0.8
2,200,650 - -0.1
2,200,694 - +1.0
2,200,694 - -0.2

Or see this region's nucleotide sequence