Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10375 and BWI76_RS10380 are separated by 13 nucleotides BWI76_RS10380 and BWI76_RS10385 are separated by 142 nucleotides BWI76_RS10385 and BWI76_RS10390 are separated by 4 nucleotides
BWI76_RS10375: BWI76_RS10375 - polar amino acid ABC transporter inner membrane subunit, at 2,196,857 to 2,197,522
_RS10375
BWI76_RS10380: BWI76_RS10380 - polar amino acid ABC transporter inner membrane subunit, at 2,197,536 to 2,198,207
_RS10380
BWI76_RS10385: BWI76_RS10385 - MmgE/PrpD family protein, at 2,198,350 to 2,199,759
_RS10385
BWI76_RS10390: BWI76_RS10390 - amino acid ABC transporter, at 2,199,764 to 2,200,588
_RS10390
Position (kb)
2198
2199
2200 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2197.551 kb on - strand at 2197.617 kb on - strand, within BWI76_RS10380 at 2197.683 kb on - strand, within BWI76_RS10380 at 2198.137 kb on + strand, within BWI76_RS10380 at 2198.140 kb on + strand at 2198.209 kb on + strand at 2198.262 kb on + strand at 2198.433 kb on + strand at 2198.606 kb on - strand, within BWI76_RS10385 at 2198.832 kb on - strand, within BWI76_RS10385 at 2199.436 kb on - strand, within BWI76_RS10385 at 2199.577 kb on + strand, within BWI76_RS10385 at 2199.805 kb on + strand at 2199.813 kb on - strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.828 kb on - strand at 2199.828 kb on - strand at 2199.828 kb on - strand at 2199.828 kb on - strand at 2200.149 kb on - strand, within BWI76_RS10390 at 2200.233 kb on + strand, within BWI76_RS10390 at 2200.235 kb on - strand, within BWI76_RS10390 at 2200.299 kb on + strand, within BWI76_RS10390 at 2200.307 kb on - strand, within BWI76_RS10390 at 2200.307 kb on - strand, within BWI76_RS10390 at 2200.307 kb on - strand, within BWI76_RS10390 at 2200.337 kb on + strand, within BWI76_RS10390 at 2200.341 kb on - strand, within BWI76_RS10390 at 2200.371 kb on - strand, within BWI76_RS10390 at 2200.387 kb on + strand, within BWI76_RS10390 at 2200.398 kb on + strand, within BWI76_RS10390 at 2200.399 kb on + strand at 2200.407 kb on - strand, within BWI76_RS10390 at 2200.408 kb on - strand, within BWI76_RS10390 at 2200.408 kb on - strand, within BWI76_RS10390 at 2200.413 kb on - strand, within BWI76_RS10390 at 2200.413 kb on - strand, within BWI76_RS10390 at 2200.438 kb on - strand, within BWI76_RS10390 at 2200.452 kb on + strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.465 kb on - strand, within BWI76_RS10390 at 2200.465 kb on - strand, within BWI76_RS10390 at 2200.465 kb on - strand at 2200.467 kb on + strand, within BWI76_RS10390 at 2200.470 kb on - strand, within BWI76_RS10390 at 2200.470 kb on - strand, within BWI76_RS10390 at 2200.475 kb on - strand, within BWI76_RS10390 at 2200.475 kb on - strand, within BWI76_RS10390 at 2200.514 kb on + strand at 2200.522 kb on - strand at 2200.548 kb on + strand at 2200.598 kb on - strand at 2200.650 kb on - strand at 2200.650 kb on - strand at 2200.694 kb on - strand at 2200.694 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 2,197,551 - +0.3 2,197,617 - BWI76_RS10380 0.12 -0.4 2,197,683 - BWI76_RS10380 0.22 -0.2 2,198,137 + BWI76_RS10380 0.89 +0.0 2,198,140 + +0.4 2,198,209 + -0.3 2,198,262 + +0.4 2,198,433 + -0.2 2,198,606 - BWI76_RS10385 0.18 -0.2 2,198,832 - BWI76_RS10385 0.34 -0.4 2,199,436 - BWI76_RS10385 0.77 -2.3 2,199,577 + BWI76_RS10385 0.87 -1.3 2,199,805 + +0.2 2,199,813 - +0.2 2,199,820 + +0.8 2,199,820 + +1.1 2,199,820 + -0.4 2,199,820 + +0.5 2,199,820 + -0.3 2,199,820 + -2.7 2,199,828 - +0.2 2,199,828 - -0.1 2,199,828 - +0.0 2,199,828 - -0.2 2,200,149 - BWI76_RS10390 0.47 -0.1 2,200,233 + BWI76_RS10390 0.57 -0.5 2,200,235 - BWI76_RS10390 0.57 -1.0 2,200,299 + BWI76_RS10390 0.65 +0.4 2,200,307 - BWI76_RS10390 0.66 +0.3 2,200,307 - BWI76_RS10390 0.66 +0.1 2,200,307 - BWI76_RS10390 0.66 -0.0 2,200,337 + BWI76_RS10390 0.69 -0.8 2,200,341 - BWI76_RS10390 0.70 +0.3 2,200,371 - BWI76_RS10390 0.74 -2.7 2,200,387 + BWI76_RS10390 0.76 -0.9 2,200,398 + BWI76_RS10390 0.77 -0.1 2,200,399 + -0.1 2,200,407 - BWI76_RS10390 0.78 +0.8 2,200,408 - BWI76_RS10390 0.78 +1.1 2,200,408 - BWI76_RS10390 0.78 -0.7 2,200,413 - BWI76_RS10390 0.79 -0.1 2,200,413 - BWI76_RS10390 0.79 +0.1 2,200,438 - BWI76_RS10390 0.82 -0.3 2,200,452 + BWI76_RS10390 0.83 +0.7 2,200,460 - BWI76_RS10390 0.84 -0.3 2,200,460 - BWI76_RS10390 0.84 -0.1 2,200,460 - BWI76_RS10390 0.84 -0.1 2,200,460 - BWI76_RS10390 0.84 +0.0 2,200,460 - BWI76_RS10390 0.84 +0.7 2,200,460 - BWI76_RS10390 0.84 -0.4 2,200,460 - BWI76_RS10390 0.84 -0.7 2,200,465 - BWI76_RS10390 0.85 +0.1 2,200,465 - BWI76_RS10390 0.85 -1.0 2,200,465 - -0.7 2,200,467 + BWI76_RS10390 0.85 -2.4 2,200,470 - BWI76_RS10390 0.86 -0.9 2,200,470 - BWI76_RS10390 0.86 -0.9 2,200,475 - BWI76_RS10390 0.86 +0.1 2,200,475 - BWI76_RS10390 0.86 -0.4 2,200,514 + +1.1 2,200,522 - +1.4 2,200,548 + +2.1 2,200,598 - +0.3 2,200,650 - -0.8 2,200,650 - -0.1 2,200,694 - +1.0 2,200,694 - -0.2
Or see this region's nucleotide sequence