Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24905 and BWI76_RS24910 are separated by 4 nucleotides BWI76_RS24910 and BWI76_RS24915 are separated by 63 nucleotides BWI76_RS24915 and BWI76_RS24920 overlap by 43 nucleotides BWI76_RS24920 and BWI76_RS24925 are separated by 22 nucleotides BWI76_RS24925 and BWI76_RS24930 are separated by 9 nucleotides
BWI76_RS24905: BWI76_RS24905 - filamentation induced by cAMP protein fic, at 5,189,505 to 5,190,506
_RS24905
BWI76_RS24910: BWI76_RS24910 - rRNA (cytidine-2'-O-)-methyltransferase, at 5,190,511 to 5,191,374
_RS24910
BWI76_RS24915: BWI76_RS24915 - penicillin-binding protein activator, at 5,191,438 to 5,193,519
_RS24915
BWI76_RS24920: BWI76_RS24920 - YraN family protein, at 5,193,477 to 5,193,863
_RS24920
BWI76_RS24925: BWI76_RS24925 - DnaA initiator-associating protein DiaA, at 5,193,886 to 5,194,476
_RS24925
BWI76_RS24930: BWI76_RS24930 - osmotically-inducible protein OsmY, at 5,194,486 to 5,195,061
_RS24930
Position (kb)
5191
5192
5193
5194 Strain fitness (log2 ratio)
-1
0
1
2 at 5190.468 kb on + strand at 5190.481 kb on - strand at 5190.508 kb on + strand at 5190.517 kb on - strand at 5190.523 kb on - strand at 5190.536 kb on + strand at 5190.536 kb on + strand at 5190.536 kb on + strand at 5190.544 kb on - strand at 5190.544 kb on - strand at 5190.699 kb on - strand, within BWI76_RS24910 at 5190.911 kb on - strand, within BWI76_RS24910 at 5191.009 kb on + strand, within BWI76_RS24910 at 5191.010 kb on + strand, within BWI76_RS24910 at 5191.125 kb on - strand, within BWI76_RS24910 at 5191.125 kb on - strand, within BWI76_RS24910 at 5191.127 kb on + strand, within BWI76_RS24910 at 5191.135 kb on - strand, within BWI76_RS24910 at 5191.275 kb on + strand, within BWI76_RS24910 at 5191.283 kb on - strand, within BWI76_RS24910 at 5191.319 kb on - strand at 5191.336 kb on - strand at 5191.336 kb on - strand at 5191.721 kb on - strand, within BWI76_RS24915 at 5191.935 kb on - strand, within BWI76_RS24915 at 5191.978 kb on + strand, within BWI76_RS24915 at 5192.049 kb on - strand, within BWI76_RS24915 at 5192.064 kb on - strand, within BWI76_RS24915 at 5192.195 kb on - strand, within BWI76_RS24915 at 5192.334 kb on + strand, within BWI76_RS24915 at 5192.431 kb on + strand, within BWI76_RS24915 at 5192.563 kb on - strand, within BWI76_RS24915 at 5192.681 kb on - strand, within BWI76_RS24915 at 5192.703 kb on + strand, within BWI76_RS24915 at 5192.833 kb on + strand, within BWI76_RS24915 at 5193.028 kb on + strand, within BWI76_RS24915 at 5193.028 kb on + strand, within BWI76_RS24915 at 5193.028 kb on + strand, within BWI76_RS24915 at 5193.036 kb on - strand, within BWI76_RS24915 at 5193.040 kb on + strand, within BWI76_RS24915 at 5193.040 kb on + strand, within BWI76_RS24915 at 5193.041 kb on - strand, within BWI76_RS24915 at 5193.048 kb on - strand, within BWI76_RS24915 at 5193.158 kb on - strand, within BWI76_RS24915 at 5193.170 kb on + strand, within BWI76_RS24915 at 5193.170 kb on + strand, within BWI76_RS24915 at 5193.172 kb on + strand, within BWI76_RS24915 at 5193.207 kb on - strand, within BWI76_RS24915 at 5193.323 kb on + strand at 5193.408 kb on - strand at 5193.412 kb on + strand at 5193.445 kb on + strand at 5193.636 kb on + strand, within BWI76_RS24920 at 5193.745 kb on - strand, within BWI76_RS24920 at 5193.807 kb on + strand, within BWI76_RS24920 at 5194.007 kb on - strand, within BWI76_RS24925 at 5194.186 kb on + strand, within BWI76_RS24925 at 5194.314 kb on - strand, within BWI76_RS24925 at 5194.493 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X remove 5,190,468 + -0.4 5,190,481 - -0.6 5,190,508 + +0.0 5,190,517 - -0.3 5,190,523 - -0.7 5,190,536 + -0.2 5,190,536 + +0.0 5,190,536 + +0.0 5,190,544 - -0.0 5,190,544 - +0.7 5,190,699 - BWI76_RS24910 0.22 -0.1 5,190,911 - BWI76_RS24910 0.46 -0.7 5,191,009 + BWI76_RS24910 0.58 -0.0 5,191,010 + BWI76_RS24910 0.58 -0.8 5,191,125 - BWI76_RS24910 0.71 +0.2 5,191,125 - BWI76_RS24910 0.71 +0.4 5,191,127 + BWI76_RS24910 0.71 +0.3 5,191,135 - BWI76_RS24910 0.72 +0.3 5,191,275 + BWI76_RS24910 0.88 -1.6 5,191,283 - BWI76_RS24910 0.89 +0.2 5,191,319 - +0.3 5,191,336 - -0.3 5,191,336 - -0.1 5,191,721 - BWI76_RS24915 0.14 +0.6 5,191,935 - BWI76_RS24915 0.24 +0.4 5,191,978 + BWI76_RS24915 0.26 -0.9 5,192,049 - BWI76_RS24915 0.29 +0.5 5,192,064 - BWI76_RS24915 0.30 +0.5 5,192,195 - BWI76_RS24915 0.36 -0.8 5,192,334 + BWI76_RS24915 0.43 -0.0 5,192,431 + BWI76_RS24915 0.48 +0.4 5,192,563 - BWI76_RS24915 0.54 +0.8 5,192,681 - BWI76_RS24915 0.60 +0.5 5,192,703 + BWI76_RS24915 0.61 +0.3 5,192,833 + BWI76_RS24915 0.67 +0.0 5,193,028 + BWI76_RS24915 0.76 +1.1 5,193,028 + BWI76_RS24915 0.76 +0.3 5,193,028 + BWI76_RS24915 0.76 -0.3 5,193,036 - BWI76_RS24915 0.77 -0.5 5,193,040 + BWI76_RS24915 0.77 +0.2 5,193,040 + BWI76_RS24915 0.77 +0.7 5,193,041 - BWI76_RS24915 0.77 +0.4 5,193,048 - BWI76_RS24915 0.77 +0.7 5,193,158 - BWI76_RS24915 0.83 +0.4 5,193,170 + BWI76_RS24915 0.83 +0.7 5,193,170 + BWI76_RS24915 0.83 +2.1 5,193,172 + BWI76_RS24915 0.83 +0.3 5,193,207 - BWI76_RS24915 0.85 +0.7 5,193,323 + +0.9 5,193,408 - +1.6 5,193,412 + +0.8 5,193,445 + +0.2 5,193,636 + BWI76_RS24920 0.41 -0.9 5,193,745 - BWI76_RS24920 0.69 +1.6 5,193,807 + BWI76_RS24920 0.85 -0.4 5,194,007 - BWI76_RS24925 0.20 +1.0 5,194,186 + BWI76_RS24925 0.51 -0.4 5,194,314 - BWI76_RS24925 0.72 -1.6 5,194,493 - +0.2
Or see this region's nucleotide sequence