Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16475 and BWI76_RS16480 are separated by 79 nucleotides BWI76_RS16480 and BWI76_RS16485 are separated by 106 nucleotides BWI76_RS16485 and BWI76_RS16490 are separated by 51 nucleotides BWI76_RS16490 and BWI76_RS16495 are separated by 339 nucleotides
BWI76_RS16475: BWI76_RS16475 - alkene reductase, at 3,416,096 to 3,417,193
_RS16475
BWI76_RS16480: BWI76_RS16480 - lactoylglutathione lyase, at 3,417,273 to 3,417,680
_RS16480
BWI76_RS16485: BWI76_RS16485 - ribonuclease T, at 3,417,787 to 3,418,431
_RS16485
BWI76_RS16490: BWI76_RS16490 - glutaredoxin, at 3,418,483 to 3,418,830
_RS16490
BWI76_RS16495: BWI76_RS16495 - endopeptidase, at 3,419,170 to 3,420,024
_RS16495
Position (kb) 3417
3418
3419 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3417.036 kb on + strand, within BWI76_RS16475 at 3417.229 kb on + strand at 3417.237 kb on - strand at 3417.334 kb on + strand, within BWI76_RS16480 at 3417.476 kb on + strand, within BWI76_RS16480 at 3417.527 kb on - strand, within BWI76_RS16480 at 3417.658 kb on + strand at 3417.804 kb on + strand at 3418.251 kb on - strand, within BWI76_RS16485 at 3418.312 kb on + strand, within BWI76_RS16485 at 3418.612 kb on - strand, within BWI76_RS16490 at 3419.134 kb on + strand at 3419.134 kb on + strand at 3419.306 kb on - strand, within BWI76_RS16495
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X remove 3,417,036 + BWI76_RS16475 0.86 -2.9 3,417,229 + -0.5 3,417,237 - -0.6 3,417,334 + BWI76_RS16480 0.15 +0.6 3,417,476 + BWI76_RS16480 0.50 -0.4 3,417,527 - BWI76_RS16480 0.62 +0.4 3,417,658 + -0.1 3,417,804 + -3.6 3,418,251 - BWI76_RS16485 0.72 -1.0 3,418,312 + BWI76_RS16485 0.81 -3.4 3,418,612 - BWI76_RS16490 0.37 +0.2 3,419,134 + +0.0 3,419,134 + +1.1 3,419,306 - BWI76_RS16495 0.16 -1.1
Or see this region's nucleotide sequence