Experiment: P. phyto corn MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28285 and BPHYT_RS28290 are separated by 382 nucleotides BPHYT_RS28290 and BPHYT_RS28295 are separated by 59 nucleotides BPHYT_RS28295 and BPHYT_RS28300 are separated by 811 nucleotides
BPHYT_RS28285: BPHYT_RS28285 - MFS transporter, at 1,901,509 to 1,902,756
_RS28285
BPHYT_RS28290: BPHYT_RS28290 - hypothetical protein, at 1,903,139 to 1,903,411
_RS28290
BPHYT_RS28295: BPHYT_RS28295 - hypothetical protein, at 1,903,471 to 1,903,788
_RS28295
BPHYT_RS28300: BPHYT_RS28300 - histone nucleoid-structuring protein H-NS, at 1,904,600 to 1,904,902
_RS28300
Position (kb)
1903
1904 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1902.756 kb on + strand at 1902.756 kb on + strand at 1902.757 kb on - strand at 1902.757 kb on - strand at 1902.759 kb on + strand at 1902.774 kb on + strand at 1902.774 kb on + strand at 1902.778 kb on - strand at 1902.778 kb on - strand at 1902.932 kb on + strand at 1902.932 kb on + strand at 1902.991 kb on - strand at 1903.145 kb on - strand at 1903.145 kb on - strand at 1903.205 kb on + strand, within BPHYT_RS28290 at 1903.206 kb on - strand, within BPHYT_RS28290 at 1903.484 kb on + strand at 1903.774 kb on + strand at 1903.774 kb on + strand at 1903.781 kb on - strand at 1903.781 kb on - strand at 1903.921 kb on + strand at 1904.034 kb on + strand at 1904.035 kb on - strand at 1904.102 kb on + strand at 1904.175 kb on - strand at 1904.205 kb on - strand at 1904.420 kb on + strand at 1904.494 kb on + strand at 1904.535 kb on - strand at 1904.722 kb on + strand, within BPHYT_RS28300
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto corn MOI 1 remove 1,902,756 + +0.1 1,902,756 + -0.3 1,902,757 - -0.8 1,902,757 - -1.3 1,902,759 + +0.7 1,902,774 + +0.0 1,902,774 + -0.2 1,902,778 - +0.8 1,902,778 - -0.2 1,902,932 + -0.8 1,902,932 + +1.0 1,902,991 - -2.7 1,903,145 - -1.7 1,903,145 - -0.0 1,903,205 + BPHYT_RS28290 0.24 +0.1 1,903,206 - BPHYT_RS28290 0.25 +0.1 1,903,484 + +0.7 1,903,774 + +0.2 1,903,774 + -0.2 1,903,781 - -0.1 1,903,781 - +1.1 1,903,921 + +0.1 1,904,034 + -0.2 1,904,035 - -0.3 1,904,102 + +0.9 1,904,175 - +0.1 1,904,205 - -0.2 1,904,420 + -0.3 1,904,494 + +0.8 1,904,535 - +2.7 1,904,722 + BPHYT_RS28300 0.40 -3.3
Or see this region's nucleotide sequence