Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24505

Experiment: P. phyto corn MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24500 and BPHYT_RS24505 overlap by 4 nucleotidesBPHYT_RS24505 and BPHYT_RS24510 are separated by 54 nucleotidesBPHYT_RS24510 and BPHYT_RS24515 are separated by 267 nucleotides BPHYT_RS24500: BPHYT_RS24500 - histidine kinase, at 1,064,744 to 1,066,144 _RS24500 BPHYT_RS24505: BPHYT_RS24505 - glyoxalase, at 1,066,141 to 1,066,566 _RS24505 BPHYT_RS24510: BPHYT_RS24510 - alkylphosphonate utilization protein, at 1,066,621 to 1,066,962 _RS24510 BPHYT_RS24515: BPHYT_RS24515 - antibiotic biosynthesis monooxygenase, at 1,067,230 to 1,067,532 _RS24515 Position (kb) 1066 1067Strain fitness (log2 ratio) -1 0 1 2at 1065.473 kb on + strand, within BPHYT_RS24500at 1065.587 kb on + strand, within BPHYT_RS24500at 1066.142 kb on + strandat 1066.230 kb on - strand, within BPHYT_RS24505at 1066.242 kb on - strand, within BPHYT_RS24505at 1066.512 kb on - strand, within BPHYT_RS24505at 1066.586 kb on + strandat 1066.898 kb on + strand, within BPHYT_RS24510at 1066.898 kb on + strand, within BPHYT_RS24510at 1066.899 kb on - strand, within BPHYT_RS24510at 1067.116 kb on + strandat 1067.134 kb on + strandat 1067.135 kb on - strandat 1067.135 kb on - strandat 1067.144 kb on + strandat 1067.249 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto corn MOI 1
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1,065,473 + BPHYT_RS24500 0.52 +1.8
1,065,587 + BPHYT_RS24500 0.60 +0.0
1,066,142 + -0.2
1,066,230 - BPHYT_RS24505 0.21 -1.6
1,066,242 - BPHYT_RS24505 0.24 -0.5
1,066,512 - BPHYT_RS24505 0.87 -0.1
1,066,586 + -0.6
1,066,898 + BPHYT_RS24510 0.81 -0.4
1,066,898 + BPHYT_RS24510 0.81 -0.4
1,066,899 - BPHYT_RS24510 0.81 +0.2
1,067,116 + -0.8
1,067,134 + +1.1
1,067,135 - +0.3
1,067,135 - -1.1
1,067,144 + +0.2
1,067,249 - -1.2

Or see this region's nucleotide sequence