Experiment: P. phyto corn MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13435 and BPHYT_RS13440 are separated by 30 nucleotides BPHYT_RS13440 and BPHYT_RS13445 are separated by 99 nucleotides BPHYT_RS13445 and BPHYT_RS13450 are separated by 60 nucleotides BPHYT_RS13450 and BPHYT_RS13455 are separated by 297 nucleotides
BPHYT_RS13435: BPHYT_RS13435 - aliphatic nitrilase, at 3,067,399 to 3,068,433
_RS13435
BPHYT_RS13440: BPHYT_RS13440 - hypothetical protein, at 3,068,464 to 3,068,946
_RS13440
BPHYT_RS13445: BPHYT_RS13445 - hypothetical protein, at 3,069,046 to 3,069,246
_RS13445
BPHYT_RS13450: BPHYT_RS13450 - GntR family transcriptional regulator, at 3,069,307 to 3,070,698
_RS13450
BPHYT_RS13455: BPHYT_RS13455 - methyl-accepting chemotaxis protein, at 3,070,996 to 3,072,795
_RS13455
Position (kb)
3069
3070
3071 Strain fitness (log2 ratio)
-1
0
1 at 3068.829 kb on + strand, within BPHYT_RS13440 at 3070.184 kb on - strand, within BPHYT_RS13450 at 3070.737 kb on - strand at 3070.874 kb on + strand at 3070.906 kb on + strand at 3071.080 kb on + strand at 3071.278 kb on + strand, within BPHYT_RS13455 at 3071.418 kb on - strand, within BPHYT_RS13455 at 3071.433 kb on - strand, within BPHYT_RS13455
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto corn MOI 1 remove 3,068,829 + BPHYT_RS13440 0.76 +0.4 3,070,184 - BPHYT_RS13450 0.63 -1.7 3,070,737 - +0.4 3,070,874 + +1.1 3,070,906 + +0.6 3,071,080 + -0.4 3,071,278 + BPHYT_RS13455 0.16 -0.6 3,071,418 - BPHYT_RS13455 0.23 -1.0 3,071,433 - BPHYT_RS13455 0.24 +0.0
Or see this region's nucleotide sequence