Experiment: P. phyto corn MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04660 and BPHYT_RS04665 are separated by 15 nucleotides BPHYT_RS04665 and BPHYT_RS04670 are separated by 425 nucleotides BPHYT_RS04670 and BPHYT_RS04675 are separated by 57 nucleotides
BPHYT_RS04660: BPHYT_RS04660 - hypothetical protein, at 1,078,520 to 1,079,851
_RS04660
BPHYT_RS04665: BPHYT_RS04665 - hypothetical protein, at 1,079,867 to 1,080,334
_RS04665
BPHYT_RS04670: BPHYT_RS04670 - sulfurtransferase, at 1,080,760 to 1,081,083
_RS04670
BPHYT_RS04675: BPHYT_RS04675 - protein-L-isoaspartate O-methyltransferase, at 1,081,141 to 1,081,794
_RS04675
Position (kb)
1080
1081
1082 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1079.913 kb on - strand at 1079.913 kb on - strand at 1080.120 kb on + strand, within BPHYT_RS04665 at 1080.221 kb on - strand, within BPHYT_RS04665 at 1080.682 kb on - strand at 1080.685 kb on - strand at 1080.685 kb on - strand at 1080.765 kb on - strand at 1081.030 kb on - strand, within BPHYT_RS04670 at 1081.190 kb on + strand at 1081.190 kb on + strand at 1081.190 kb on + strand at 1081.191 kb on - strand at 1081.191 kb on - strand at 1081.191 kb on - strand at 1081.191 kb on - strand at 1081.365 kb on - strand, within BPHYT_RS04675 at 1081.527 kb on - strand, within BPHYT_RS04675 at 1081.670 kb on + strand, within BPHYT_RS04675 at 1081.809 kb on + strand at 1081.810 kb on - strand at 1081.923 kb on - strand at 1082.040 kb on - strand at 1082.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto corn MOI 1 remove 1,079,913 - -1.7 1,079,913 - -0.3 1,080,120 + BPHYT_RS04665 0.54 +0.6 1,080,221 - BPHYT_RS04665 0.76 +0.4 1,080,682 - -1.3 1,080,685 - -1.5 1,080,685 - +0.1 1,080,765 - +0.1 1,081,030 - BPHYT_RS04670 0.83 +0.6 1,081,190 + +2.8 1,081,190 + -0.3 1,081,190 + -0.4 1,081,191 - -0.1 1,081,191 - +1.2 1,081,191 - +0.3 1,081,191 - +0.3 1,081,365 - BPHYT_RS04675 0.34 -0.2 1,081,527 - BPHYT_RS04675 0.59 -0.4 1,081,670 + BPHYT_RS04675 0.81 -0.8 1,081,809 + -0.1 1,081,810 - -0.2 1,081,923 - -1.0 1,082,040 - +0.8 1,082,040 - +1.4
Or see this region's nucleotide sequence