Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS26000

Experiment: P. phyto PHYTO2 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25995 and BPHYT_RS26000 are separated by 9 nucleotidesBPHYT_RS26000 and BPHYT_RS26005 are separated by 156 nucleotides BPHYT_RS25995: BPHYT_RS25995 - 2OG-Fe(II) oxygenase, at 1,408,690 to 1,409,745 _RS25995 BPHYT_RS26000: BPHYT_RS26000 - xylose isomerase, at 1,409,755 to 1,410,657 _RS26000 BPHYT_RS26005: BPHYT_RS26005 - MarR family transcriptional regulator, at 1,410,814 to 1,411,182 _RS26005 Position (kb) 1409 1410 1411Strain fitness (log2 ratio) -1 0 1at 1408.768 kb on + strandat 1408.769 kb on - strandat 1408.769 kb on - strandat 1408.799 kb on + strand, within BPHYT_RS25995at 1408.913 kb on + strand, within BPHYT_RS25995at 1409.310 kb on - strand, within BPHYT_RS25995at 1409.514 kb on - strand, within BPHYT_RS25995at 1409.612 kb on + strand, within BPHYT_RS25995at 1409.612 kb on + strand, within BPHYT_RS25995at 1409.868 kb on + strand, within BPHYT_RS26000at 1409.868 kb on + strand, within BPHYT_RS26000at 1409.869 kb on - strand, within BPHYT_RS26000at 1409.869 kb on - strand, within BPHYT_RS26000at 1409.925 kb on - strand, within BPHYT_RS26000at 1410.461 kb on + strand, within BPHYT_RS26000at 1410.728 kb on + strandat 1410.780 kb on + strandat 1410.967 kb on + strand, within BPHYT_RS26005at 1411.264 kb on - strandat 1411.439 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 0.1
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1,408,768 + +0.0
1,408,769 - -0.8
1,408,769 - +0.0
1,408,799 + BPHYT_RS25995 0.10 -0.2
1,408,913 + BPHYT_RS25995 0.21 -0.2
1,409,310 - BPHYT_RS25995 0.59 +0.2
1,409,514 - BPHYT_RS25995 0.78 +0.2
1,409,612 + BPHYT_RS25995 0.87 +0.6
1,409,612 + BPHYT_RS25995 0.87 +1.0
1,409,868 + BPHYT_RS26000 0.13 -1.2
1,409,868 + BPHYT_RS26000 0.13 -0.6
1,409,869 - BPHYT_RS26000 0.13 -1.0
1,409,869 - BPHYT_RS26000 0.13 -0.5
1,409,925 - BPHYT_RS26000 0.19 -0.7
1,410,461 + BPHYT_RS26000 0.78 -1.2
1,410,728 + +0.2
1,410,780 + +0.3
1,410,967 + BPHYT_RS26005 0.41 +0.1
1,411,264 - +0.7
1,411,439 + +0.7

Or see this region's nucleotide sequence