Experiment: P. phyto PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21785 and BPHYT_RS21790 are separated by 88 nucleotides BPHYT_RS21790 and BPHYT_RS21795 are separated by 407 nucleotides BPHYT_RS21795 and BPHYT_RS21800 are separated by 39 nucleotides BPHYT_RS21800 and BPHYT_RS21805 are separated by 200 nucleotides
BPHYT_RS21785: BPHYT_RS21785 - hypothetical protein, at 444,824 to 445,156
_RS21785
BPHYT_RS21790: BPHYT_RS21790 - hypothetical protein, at 445,245 to 445,583
_RS21790
BPHYT_RS21795: BPHYT_RS21795 - membrane protein, at 445,991 to 447,550
_RS21795
BPHYT_RS21800: BPHYT_RS21800 - hypothetical protein, at 447,590 to 447,892
_RS21800
BPHYT_RS21805: BPHYT_RS21805 - transcriptional regulator, at 448,093 to 448,773
_RS21805
Position (kb) 445
446
447
448 Strain fitness (log2 ratio) -1
0
1
2
3 at 445.117 kb on + strand, within BPHYT_RS21785 at 445.146 kb on - strand at 445.289 kb on + strand, within BPHYT_RS21790 at 445.290 kb on - strand, within BPHYT_RS21790 at 445.570 kb on - strand at 445.711 kb on - strand at 445.858 kb on + strand at 448.247 kb on + strand, within BPHYT_RS21805 at 448.248 kb on - strand, within BPHYT_RS21805 at 448.248 kb on - strand, within BPHYT_RS21805
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 0.1 remove 445,117 + BPHYT_RS21785 0.88 +3.1 445,146 - -1.3 445,289 + BPHYT_RS21790 0.13 +0.2 445,290 - BPHYT_RS21790 0.13 -0.3 445,570 - -0.1 445,711 - +0.0 445,858 + -0.0 448,247 + BPHYT_RS21805 0.23 -0.1 448,248 - BPHYT_RS21805 0.23 +0.5 448,248 - BPHYT_RS21805 0.23 -0.7
Or see this region's nucleotide sequence