Experiment: P. phyto PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21260 and BPHYT_RS21265 overlap by 1 nucleotides BPHYT_RS21265 and BPHYT_RS21270 are separated by 57 nucleotides BPHYT_RS21270 and BPHYT_RS21275 are separated by 105 nucleotides BPHYT_RS21275 and BPHYT_RS21280 are separated by 216 nucleotides
BPHYT_RS21260: BPHYT_RS21260 - ABC transporter permease, at 330,662 to 331,540
_RS21260
BPHYT_RS21265: BPHYT_RS21265 - ABC transporter permease, at 331,540 to 332,478
_RS21265
BPHYT_RS21270: BPHYT_RS21270 - dihydroxy-acid dehydratase, at 332,536 to 334,296
_RS21270
BPHYT_RS21275: BPHYT_RS21275 - short-chain dehydrogenase, at 334,402 to 335,007
_RS21275
BPHYT_RS21280: BPHYT_RS21280 - hypothetical protein, at 335,224 to 335,676
_RS21280
Position (kb) 332
333
334
335 Strain fitness (log2 ratio) -1
0
1
2
3 at 331.807 kb on + strand, within BPHYT_RS21265 at 331.808 kb on - strand, within BPHYT_RS21265 at 332.026 kb on + strand, within BPHYT_RS21265 at 332.027 kb on - strand, within BPHYT_RS21265 at 332.027 kb on - strand, within BPHYT_RS21265 at 332.027 kb on - strand, within BPHYT_RS21265 at 332.027 kb on - strand, within BPHYT_RS21265 at 332.191 kb on + strand, within BPHYT_RS21265 at 332.323 kb on + strand, within BPHYT_RS21265 at 332.323 kb on + strand, within BPHYT_RS21265 at 332.324 kb on - strand, within BPHYT_RS21265 at 332.326 kb on + strand, within BPHYT_RS21265 at 332.326 kb on + strand, within BPHYT_RS21265 at 332.326 kb on + strand, within BPHYT_RS21265 at 332.386 kb on + strand at 332.386 kb on + strand at 332.387 kb on - strand at 332.387 kb on - strand at 332.388 kb on + strand at 332.654 kb on - strand at 333.378 kb on + strand, within BPHYT_RS21270 at 333.485 kb on - strand, within BPHYT_RS21270 at 333.779 kb on - strand, within BPHYT_RS21270 at 333.779 kb on - strand, within BPHYT_RS21270 at 333.896 kb on + strand, within BPHYT_RS21270 at 333.977 kb on - strand, within BPHYT_RS21270 at 334.273 kb on - strand at 334.514 kb on + strand, within BPHYT_RS21275 at 334.515 kb on - strand, within BPHYT_RS21275 at 334.515 kb on - strand, within BPHYT_RS21275 at 334.706 kb on + strand, within BPHYT_RS21275 at 334.707 kb on - strand, within BPHYT_RS21275 at 335.009 kb on + strand at 335.010 kb on - strand at 335.010 kb on - strand at 335.157 kb on - strand at 335.166 kb on - strand at 335.166 kb on - strand at 335.272 kb on - strand, within BPHYT_RS21280
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 0.1 remove 331,807 + BPHYT_RS21265 0.28 +0.8 331,808 - BPHYT_RS21265 0.29 -0.7 332,026 + BPHYT_RS21265 0.52 +1.1 332,027 - BPHYT_RS21265 0.52 -0.2 332,027 - BPHYT_RS21265 0.52 -0.6 332,027 - BPHYT_RS21265 0.52 +0.6 332,027 - BPHYT_RS21265 0.52 -0.6 332,191 + BPHYT_RS21265 0.69 +0.2 332,323 + BPHYT_RS21265 0.83 -0.5 332,323 + BPHYT_RS21265 0.83 -0.6 332,324 - BPHYT_RS21265 0.83 +0.2 332,326 + BPHYT_RS21265 0.84 -0.1 332,326 + BPHYT_RS21265 0.84 -0.3 332,326 + BPHYT_RS21265 0.84 +0.5 332,386 + +0.5 332,386 + +0.0 332,387 - +0.6 332,387 - -0.5 332,388 + +1.0 332,654 - -0.3 333,378 + BPHYT_RS21270 0.48 -0.4 333,485 - BPHYT_RS21270 0.54 -0.9 333,779 - BPHYT_RS21270 0.71 -1.4 333,779 - BPHYT_RS21270 0.71 -0.9 333,896 + BPHYT_RS21270 0.77 -0.0 333,977 - BPHYT_RS21270 0.82 -0.1 334,273 - +0.6 334,514 + BPHYT_RS21275 0.18 +0.2 334,515 - BPHYT_RS21275 0.19 +0.0 334,515 - BPHYT_RS21275 0.19 -0.6 334,706 + BPHYT_RS21275 0.50 -0.7 334,707 - BPHYT_RS21275 0.50 +2.7 335,009 + +0.4 335,010 - -0.1 335,010 - -0.1 335,157 - +1.7 335,166 - +2.2 335,166 - +3.2 335,272 - BPHYT_RS21280 0.11 -0.3
Or see this region's nucleotide sequence