Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12270

Experiment: P. phyto PHYTO2 MOI 0.1

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBPHYT_RS12260 and BPHYT_RS12265 overlap by 14 nucleotidesBPHYT_RS12265 and BPHYT_RS12270 overlap by 4 nucleotidesBPHYT_RS12270 and BPHYT_RS12275 are separated by 560 nucleotides BPHYT_RS12260: BPHYT_RS12260 - DNA polymerase, at 2,800,157 to 2,801,401 _RS12260 BPHYT_RS12265: BPHYT_RS12265 - alanine acetyltransferase, at 2,801,388 to 2,801,882 _RS12265 BPHYT_RS12270: BPHYT_RS12270 - O-sialoglycoprotein endopeptidase, at 2,801,879 to 2,802,709 _RS12270 BPHYT_RS12275: BPHYT_RS12275 - acyl-CoA-binding protein, at 2,803,270 to 2,803,539 _RS12275 Position (kb) 2801 2802 2803Strain fitness (log2 ratio) -2 -1 0 1at 2801.516 kb on - strand, within BPHYT_RS12265at 2801.744 kb on - strand, within BPHYT_RS12265at 2801.853 kb on - strandat 2801.853 kb on - strandat 2801.855 kb on - strandat 2802.721 kb on + strandat 2803.086 kb on - strandat 2803.218 kb on + strandat 2803.218 kb on + strandat 2803.435 kb on + strand, within BPHYT_RS12275at 2803.435 kb on + strand, within BPHYT_RS12275at 2803.441 kb on + strand, within BPHYT_RS12275at 2803.441 kb on + strand, within BPHYT_RS12275at 2803.441 kb on + strand, within BPHYT_RS12275at 2803.495 kb on + strand, within BPHYT_RS12275at 2803.705 kb on - strandat 2803.707 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 0.1
remove
2,801,516 - BPHYT_RS12265 0.26 +0.4
2,801,744 - BPHYT_RS12265 0.72 -2.2
2,801,853 - +0.5
2,801,853 - +0.2
2,801,855 - -0.7
2,802,721 + +1.0
2,803,086 - +0.2
2,803,218 + -1.7
2,803,218 + +0.1
2,803,435 + BPHYT_RS12275 0.61 +1.3
2,803,435 + BPHYT_RS12275 0.61 -0.3
2,803,441 + BPHYT_RS12275 0.63 +0.1
2,803,441 + BPHYT_RS12275 0.63 -0.6
2,803,441 + BPHYT_RS12275 0.63 -0.2
2,803,495 + BPHYT_RS12275 0.83 +0.5
2,803,705 - -2.3
2,803,707 + -0.0

Or see this region's nucleotide sequence