Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36080 and BPHYT_RS36085 overlap by 4 nucleotides BPHYT_RS36085 and BPHYT_RS36090 are separated by 428 nucleotides BPHYT_RS36090 and BPHYT_RS36095 are separated by 19 nucleotides BPHYT_RS36095 and BPHYT_RS36100 are separated by 128 nucleotides BPHYT_RS36100 and BPHYT_RS36105 are separated by 54 nucleotides
BPHYT_RS36080: BPHYT_RS36080 - hypothetical protein, at 40,568 to 40,915
_RS36080
BPHYT_RS36085: BPHYT_RS36085 - hypothetical protein, at 40,912 to 41,403
_RS36085
BPHYT_RS36090: BPHYT_RS36090 - hypothetical protein, at 41,832 to 42,038
_RS36090
BPHYT_RS36095: BPHYT_RS36095 - hypothetical protein, at 42,058 to 42,282
_RS36095
BPHYT_RS36100: BPHYT_RS36100 - hypothetical protein, at 42,411 to 42,680
_RS36100
BPHYT_RS36105: BPHYT_RS36105 - hypothetical protein, at 42,735 to 43,067
_RS36105
Position (kb)
41
42
43 Strain fitness (log2 ratio)
-2
-1
0
1 at 40.887 kb on + strand at 41.292 kb on - strand, within BPHYT_RS36085 at 41.814 kb on - strand at 41.861 kb on + strand, within BPHYT_RS36090 at 41.861 kb on + strand, within BPHYT_RS36090 at 41.862 kb on - strand, within BPHYT_RS36090 at 42.371 kb on - strand at 42.504 kb on + strand, within BPHYT_RS36100 at 42.658 kb on - strand at 42.866 kb on - strand, within BPHYT_RS36105
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 40,887 + -0.3 41,292 - BPHYT_RS36085 0.77 +0.2 41,814 - -2.7 41,861 + BPHYT_RS36090 0.14 -2.5 41,861 + BPHYT_RS36090 0.14 -1.8 41,862 - BPHYT_RS36090 0.14 -1.6 42,371 - +0.6 42,504 + BPHYT_RS36100 0.34 +0.6 42,658 - -0.8 42,866 - BPHYT_RS36105 0.39 +0.6
Or see this region's nucleotide sequence