Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33460

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33450 and BPHYT_RS33455 are separated by 108 nucleotidesBPHYT_RS33455 and BPHYT_RS33460 are separated by 3 nucleotidesBPHYT_RS33460 and BPHYT_RS33465 are separated by 147 nucleotides BPHYT_RS33450: BPHYT_RS33450 - LysR family transcriptional regulator, at 3,091,846 to 3,092,829 _RS33450 BPHYT_RS33455: BPHYT_RS33455 - protocatechuate 3,4-dioxygenase subunit beta, at 3,092,938 to 3,093,642 _RS33455 BPHYT_RS33460: BPHYT_RS33460 - protocatechuate 3,4-dioxygenase subunit alpha, at 3,093,646 to 3,094,233 _RS33460 BPHYT_RS33465: BPHYT_RS33465 - D-alanyl-D-alanine carboxypeptidase, at 3,094,381 to 3,095,613 _RS33465 Position (kb) 3093 3094 3095Strain fitness (log2 ratio) -2 -1 0 1 2at 3092.889 kb on - strandat 3092.986 kb on + strandat 3092.987 kb on - strandat 3093.412 kb on - strand, within BPHYT_RS33455at 3093.589 kb on + strandat 3093.688 kb on + strandat 3094.066 kb on + strand, within BPHYT_RS33460at 3094.255 kb on + strandat 3094.269 kb on - strandat 3094.288 kb on + strandat 3094.570 kb on + strand, within BPHYT_RS33465at 3094.765 kb on - strand, within BPHYT_RS33465at 3094.875 kb on + strand, within BPHYT_RS33465at 3094.876 kb on - strand, within BPHYT_RS33465at 3095.159 kb on - strand, within BPHYT_RS33465

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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3,092,889 - -0.7
3,092,986 + +1.2
3,092,987 - +1.0
3,093,412 - BPHYT_RS33455 0.67 -0.5
3,093,589 + +0.3
3,093,688 + -0.1
3,094,066 + BPHYT_RS33460 0.71 -1.6
3,094,255 + -1.5
3,094,269 - -0.5
3,094,288 + +0.3
3,094,570 + BPHYT_RS33465 0.15 -2.3
3,094,765 - BPHYT_RS33465 0.31 +1.7
3,094,875 + BPHYT_RS33465 0.40 -0.7
3,094,876 - BPHYT_RS33465 0.40 +2.1
3,095,159 - BPHYT_RS33465 0.63 -1.7

Or see this region's nucleotide sequence