Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33450 and BPHYT_RS33455 are separated by 108 nucleotides BPHYT_RS33455 and BPHYT_RS33460 are separated by 3 nucleotides BPHYT_RS33460 and BPHYT_RS33465 are separated by 147 nucleotides
BPHYT_RS33450: BPHYT_RS33450 - LysR family transcriptional regulator, at 3,091,846 to 3,092,829
_RS33450
BPHYT_RS33455: BPHYT_RS33455 - protocatechuate 3,4-dioxygenase subunit beta, at 3,092,938 to 3,093,642
_RS33455
BPHYT_RS33460: BPHYT_RS33460 - protocatechuate 3,4-dioxygenase subunit alpha, at 3,093,646 to 3,094,233
_RS33460
BPHYT_RS33465: BPHYT_RS33465 - D-alanyl-D-alanine carboxypeptidase, at 3,094,381 to 3,095,613
_RS33465
Position (kb)
3093
3094
3095 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3092.889 kb on - strand at 3092.986 kb on + strand at 3092.987 kb on - strand at 3093.412 kb on - strand, within BPHYT_RS33455 at 3093.589 kb on + strand at 3093.688 kb on + strand at 3094.066 kb on + strand, within BPHYT_RS33460 at 3094.255 kb on + strand at 3094.269 kb on - strand at 3094.288 kb on + strand at 3094.570 kb on + strand, within BPHYT_RS33465 at 3094.765 kb on - strand, within BPHYT_RS33465 at 3094.875 kb on + strand, within BPHYT_RS33465 at 3094.876 kb on - strand, within BPHYT_RS33465 at 3095.159 kb on - strand, within BPHYT_RS33465
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 3,092,889 - -0.7 3,092,986 + +1.2 3,092,987 - +1.0 3,093,412 - BPHYT_RS33455 0.67 -0.5 3,093,589 + +0.3 3,093,688 + -0.1 3,094,066 + BPHYT_RS33460 0.71 -1.6 3,094,255 + -1.5 3,094,269 - -0.5 3,094,288 + +0.3 3,094,570 + BPHYT_RS33465 0.15 -2.3 3,094,765 - BPHYT_RS33465 0.31 +1.7 3,094,875 + BPHYT_RS33465 0.40 -0.7 3,094,876 - BPHYT_RS33465 0.40 +2.1 3,095,159 - BPHYT_RS33465 0.63 -1.7
Or see this region's nucleotide sequence