Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30580 and BPHYT_RS30585 are separated by 5 nucleotides BPHYT_RS30585 and BPHYT_RS30590 are separated by 114 nucleotides BPHYT_RS30590 and BPHYT_RS30595 are separated by 156 nucleotides BPHYT_RS30595 and BPHYT_RS30600 are separated by 69 nucleotides
BPHYT_RS30580: BPHYT_RS30580 - cupin, at 2,418,556 to 2,419,065
_RS30580
BPHYT_RS30585: BPHYT_RS30585 - dTDP-glucose 4,6-dehydratase, at 2,419,071 to 2,419,808
_RS30585
BPHYT_RS30590: BPHYT_RS30590 - transcriptional regulator, at 2,419,923 to 2,421,494
_RS30590
BPHYT_RS30595: BPHYT_RS30595 - membrane protein, at 2,421,651 to 2,422,112
_RS30595
BPHYT_RS30600: BPHYT_RS30600 - alpha/beta hydrolase, at 2,422,182 to 2,423,213
_RS30600
Position (kb)
2419
2420
2421
2422 Strain fitness (log2 ratio)
-2
-1
0
1 at 2419.273 kb on + strand, within BPHYT_RS30585 at 2419.274 kb on - strand, within BPHYT_RS30585 at 2419.730 kb on - strand, within BPHYT_RS30585 at 2419.853 kb on - strand at 2419.853 kb on - strand at 2419.872 kb on - strand at 2420.487 kb on + strand, within BPHYT_RS30590 at 2420.488 kb on - strand, within BPHYT_RS30590 at 2420.928 kb on + strand, within BPHYT_RS30590 at 2421.107 kb on - strand, within BPHYT_RS30590 at 2422.244 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,419,273 + BPHYT_RS30585 0.27 +0.0 2,419,274 - BPHYT_RS30585 0.28 -2.3 2,419,730 - BPHYT_RS30585 0.89 -1.5 2,419,853 - +0.3 2,419,853 - +0.6 2,419,872 - +0.3 2,420,487 + BPHYT_RS30590 0.36 -2.2 2,420,488 - BPHYT_RS30590 0.36 +1.2 2,420,928 + BPHYT_RS30590 0.64 +0.9 2,421,107 - BPHYT_RS30590 0.75 +0.1 2,422,244 + +0.2
Or see this region's nucleotide sequence