Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30590

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30580 and BPHYT_RS30585 are separated by 5 nucleotidesBPHYT_RS30585 and BPHYT_RS30590 are separated by 114 nucleotidesBPHYT_RS30590 and BPHYT_RS30595 are separated by 156 nucleotidesBPHYT_RS30595 and BPHYT_RS30600 are separated by 69 nucleotides BPHYT_RS30580: BPHYT_RS30580 - cupin, at 2,418,556 to 2,419,065 _RS30580 BPHYT_RS30585: BPHYT_RS30585 - dTDP-glucose 4,6-dehydratase, at 2,419,071 to 2,419,808 _RS30585 BPHYT_RS30590: BPHYT_RS30590 - transcriptional regulator, at 2,419,923 to 2,421,494 _RS30590 BPHYT_RS30595: BPHYT_RS30595 - membrane protein, at 2,421,651 to 2,422,112 _RS30595 BPHYT_RS30600: BPHYT_RS30600 - alpha/beta hydrolase, at 2,422,182 to 2,423,213 _RS30600 Position (kb) 2419 2420 2421 2422Strain fitness (log2 ratio) -2 -1 0 1at 2419.273 kb on + strand, within BPHYT_RS30585at 2419.274 kb on - strand, within BPHYT_RS30585at 2419.730 kb on - strand, within BPHYT_RS30585at 2419.853 kb on - strandat 2419.853 kb on - strandat 2419.872 kb on - strandat 2420.487 kb on + strand, within BPHYT_RS30590at 2420.488 kb on - strand, within BPHYT_RS30590at 2420.928 kb on + strand, within BPHYT_RS30590at 2421.107 kb on - strand, within BPHYT_RS30590at 2422.244 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,419,273 + BPHYT_RS30585 0.27 +0.0
2,419,274 - BPHYT_RS30585 0.28 -2.3
2,419,730 - BPHYT_RS30585 0.89 -1.5
2,419,853 - +0.3
2,419,853 - +0.6
2,419,872 - +0.3
2,420,487 + BPHYT_RS30590 0.36 -2.2
2,420,488 - BPHYT_RS30590 0.36 +1.2
2,420,928 + BPHYT_RS30590 0.64 +0.9
2,421,107 - BPHYT_RS30590 0.75 +0.1
2,422,244 + +0.2

Or see this region's nucleotide sequence