Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30215 and BPHYT_RS30220 are separated by 183 nucleotides BPHYT_RS30220 and BPHYT_RS30225 are separated by 55 nucleotides BPHYT_RS30225 and BPHYT_RS30230 are separated by 60 nucleotides
BPHYT_RS30215: BPHYT_RS30215 - D-2-hydroxyacid dehydrogenase, at 2,333,537 to 2,334,463
_RS30215
BPHYT_RS30220: BPHYT_RS30220 - hypothetical protein, at 2,334,647 to 2,336,044
_RS30220
BPHYT_RS30225: BPHYT_RS30225 - AsnC family transcriptional regulator, at 2,336,100 to 2,336,597
_RS30225
BPHYT_RS30230: BPHYT_RS30230 - LysR family transcriptional regulator, at 2,336,658 to 2,337,614
_RS30230
Position (kb)
2334
2335
2336
2337 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2334.577 kb on + strand at 2334.602 kb on + strand at 2334.605 kb on + strand at 2334.606 kb on - strand at 2334.606 kb on - strand at 2334.897 kb on - strand, within BPHYT_RS30220 at 2334.966 kb on + strand, within BPHYT_RS30220 at 2335.551 kb on - strand, within BPHYT_RS30220 at 2335.605 kb on - strand, within BPHYT_RS30220 at 2335.655 kb on + strand, within BPHYT_RS30220 at 2335.926 kb on - strand at 2335.983 kb on + strand at 2336.043 kb on - strand at 2336.043 kb on - strand at 2336.047 kb on + strand at 2336.097 kb on - strand at 2336.119 kb on + strand at 2336.422 kb on - strand, within BPHYT_RS30225 at 2336.427 kb on + strand, within BPHYT_RS30225 at 2336.464 kb on - strand, within BPHYT_RS30225 at 2336.524 kb on - strand, within BPHYT_RS30225
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,334,577 + -0.7 2,334,602 + -0.2 2,334,605 + -0.2 2,334,606 - +1.0 2,334,606 - -0.5 2,334,897 - BPHYT_RS30220 0.18 -1.0 2,334,966 + BPHYT_RS30220 0.23 -1.0 2,335,551 - BPHYT_RS30220 0.65 -0.1 2,335,605 - BPHYT_RS30220 0.69 -0.6 2,335,655 + BPHYT_RS30220 0.72 -0.8 2,335,926 - -0.7 2,335,983 + -0.5 2,336,043 - -0.2 2,336,043 - +1.2 2,336,047 + +0.4 2,336,097 - +2.1 2,336,119 + -0.3 2,336,422 - BPHYT_RS30225 0.65 -1.7 2,336,427 + BPHYT_RS30225 0.66 -0.2 2,336,464 - BPHYT_RS30225 0.73 -2.2 2,336,524 - BPHYT_RS30225 0.85 -1.9
Or see this region's nucleotide sequence