Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30220

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30215 and BPHYT_RS30220 are separated by 183 nucleotidesBPHYT_RS30220 and BPHYT_RS30225 are separated by 55 nucleotidesBPHYT_RS30225 and BPHYT_RS30230 are separated by 60 nucleotides BPHYT_RS30215: BPHYT_RS30215 - D-2-hydroxyacid dehydrogenase, at 2,333,537 to 2,334,463 _RS30215 BPHYT_RS30220: BPHYT_RS30220 - hypothetical protein, at 2,334,647 to 2,336,044 _RS30220 BPHYT_RS30225: BPHYT_RS30225 - AsnC family transcriptional regulator, at 2,336,100 to 2,336,597 _RS30225 BPHYT_RS30230: BPHYT_RS30230 - LysR family transcriptional regulator, at 2,336,658 to 2,337,614 _RS30230 Position (kb) 2334 2335 2336 2337Strain fitness (log2 ratio) -2 -1 0 1 2at 2334.577 kb on + strandat 2334.602 kb on + strandat 2334.605 kb on + strandat 2334.606 kb on - strandat 2334.606 kb on - strandat 2334.897 kb on - strand, within BPHYT_RS30220at 2334.966 kb on + strand, within BPHYT_RS30220at 2335.551 kb on - strand, within BPHYT_RS30220at 2335.605 kb on - strand, within BPHYT_RS30220at 2335.655 kb on + strand, within BPHYT_RS30220at 2335.926 kb on - strandat 2335.983 kb on + strandat 2336.043 kb on - strandat 2336.043 kb on - strandat 2336.047 kb on + strandat 2336.097 kb on - strandat 2336.119 kb on + strandat 2336.422 kb on - strand, within BPHYT_RS30225at 2336.427 kb on + strand, within BPHYT_RS30225at 2336.464 kb on - strand, within BPHYT_RS30225at 2336.524 kb on - strand, within BPHYT_RS30225

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,334,577 + -0.7
2,334,602 + -0.2
2,334,605 + -0.2
2,334,606 - +1.0
2,334,606 - -0.5
2,334,897 - BPHYT_RS30220 0.18 -1.0
2,334,966 + BPHYT_RS30220 0.23 -1.0
2,335,551 - BPHYT_RS30220 0.65 -0.1
2,335,605 - BPHYT_RS30220 0.69 -0.6
2,335,655 + BPHYT_RS30220 0.72 -0.8
2,335,926 - -0.7
2,335,983 + -0.5
2,336,043 - -0.2
2,336,043 - +1.2
2,336,047 + +0.4
2,336,097 - +2.1
2,336,119 + -0.3
2,336,422 - BPHYT_RS30225 0.65 -1.7
2,336,427 + BPHYT_RS30225 0.66 -0.2
2,336,464 - BPHYT_RS30225 0.73 -2.2
2,336,524 - BPHYT_RS30225 0.85 -1.9

Or see this region's nucleotide sequence