Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30065

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30060 and BPHYT_RS30065 are separated by 183 nucleotidesBPHYT_RS30065 and BPHYT_RS30070 are separated by 117 nucleotidesBPHYT_RS30070 and BPHYT_RS30075 are separated by 40 nucleotides BPHYT_RS30060: BPHYT_RS30060 - phosphoesterase, at 2,299,595 to 2,300,287 _RS30060 BPHYT_RS30065: BPHYT_RS30065 - shikimate dehydrogenase, at 2,300,471 to 2,301,319 _RS30065 BPHYT_RS30070: BPHYT_RS30070 - multidrug ABC transporter ATPase, at 2,301,437 to 2,302,060 _RS30070 BPHYT_RS30075: BPHYT_RS30075 - NADPH:quinone oxidoreductase, at 2,302,101 to 2,303,108 _RS30075 Position (kb) 2300 2301 2302Strain fitness (log2 ratio) -2 -1 0 1at 2299.611 kb on + strandat 2299.625 kb on + strandat 2299.626 kb on - strandat 2299.638 kb on + strandat 2299.675 kb on + strand, within BPHYT_RS30060at 2299.676 kb on - strand, within BPHYT_RS30060at 2299.689 kb on - strand, within BPHYT_RS30060at 2299.708 kb on - strand, within BPHYT_RS30060at 2299.862 kb on + strand, within BPHYT_RS30060at 2299.862 kb on + strand, within BPHYT_RS30060at 2299.996 kb on + strand, within BPHYT_RS30060at 2300.021 kb on + strand, within BPHYT_RS30060at 2300.118 kb on - strand, within BPHYT_RS30060at 2300.118 kb on - strand, within BPHYT_RS30060at 2300.744 kb on + strand, within BPHYT_RS30065at 2301.077 kb on - strand, within BPHYT_RS30065at 2301.356 kb on + strandat 2301.357 kb on - strandat 2301.357 kb on - strandat 2301.665 kb on + strand, within BPHYT_RS30070at 2301.666 kb on - strand, within BPHYT_RS30070at 2301.666 kb on - strand, within BPHYT_RS30070

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,299,611 + +0.8
2,299,625 + -0.5
2,299,626 - -1.7
2,299,638 + +0.1
2,299,675 + BPHYT_RS30060 0.12 +0.3
2,299,676 - BPHYT_RS30060 0.12 +0.4
2,299,689 - BPHYT_RS30060 0.14 +1.4
2,299,708 - BPHYT_RS30060 0.16 -2.0
2,299,862 + BPHYT_RS30060 0.39 -0.2
2,299,862 + BPHYT_RS30060 0.39 -0.4
2,299,996 + BPHYT_RS30060 0.58 -2.2
2,300,021 + BPHYT_RS30060 0.61 -2.2
2,300,118 - BPHYT_RS30060 0.75 -0.5
2,300,118 - BPHYT_RS30060 0.75 +0.0
2,300,744 + BPHYT_RS30065 0.32 -0.6
2,301,077 - BPHYT_RS30065 0.71 -0.3
2,301,356 + +0.6
2,301,357 - -0.7
2,301,357 - +0.7
2,301,665 + BPHYT_RS30070 0.37 +0.5
2,301,666 - BPHYT_RS30070 0.37 +0.4
2,301,666 - BPHYT_RS30070 0.37 -0.4

Or see this region's nucleotide sequence