Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29840

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29830 and BPHYT_RS29835 overlap by 8 nucleotidesBPHYT_RS29835 and BPHYT_RS29840 overlap by 4 nucleotidesBPHYT_RS29840 and BPHYT_RS29845 are separated by 38 nucleotides BPHYT_RS29830: BPHYT_RS29830 - hypothetical protein, at 2,243,330 to 2,244,637 _RS29830 BPHYT_RS29835: BPHYT_RS29835 - glycosyltransferase, at 2,244,630 to 2,245,457 _RS29835 BPHYT_RS29840: BPHYT_RS29840 - polysaccharide biosynthesis protein, at 2,245,454 to 2,246,755 _RS29840 BPHYT_RS29845: BPHYT_RS29845 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 2,246,794 to 2,248,038 _RS29845 Position (kb) 2245 2246 2247Strain fitness (log2 ratio) -1 0 1at 2245.042 kb on + strand, within BPHYT_RS29835at 2245.426 kb on - strandat 2245.904 kb on - strand, within BPHYT_RS29840at 2246.366 kb on + strand, within BPHYT_RS29840

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,245,042 + BPHYT_RS29835 0.50 +0.4
2,245,426 - -0.2
2,245,904 - BPHYT_RS29840 0.35 +0.7
2,246,366 + BPHYT_RS29840 0.70 +1.4

Or see this region's nucleotide sequence