Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29710 and BPHYT_RS29715 overlap by 4 nucleotides BPHYT_RS29715 and BPHYT_RS29720 are separated by 124 nucleotides BPHYT_RS29720 and BPHYT_RS29725 are separated by 96 nucleotides
BPHYT_RS29710: BPHYT_RS29710 - FAD-binding molybdopterin dehydrogenase, at 2,216,909 to 2,217,910
_RS29710
BPHYT_RS29715: BPHYT_RS29715 - acylaldehyde oxidase, at 2,217,907 to 2,220,129
_RS29715
BPHYT_RS29720: BPHYT_RS29720 - molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 2,220,254 to 2,220,841
_RS29720
BPHYT_RS29725: BPHYT_RS29725 - aromatic compound catabolic protein, at 2,220,938 to 2,221,486
_RS29725
Position (kb)
2217
2218
2219
2220
2221 Strain fitness (log2 ratio)
-1
0
1
2 at 2217.593 kb on + strand, within BPHYT_RS29710 at 2217.621 kb on - strand, within BPHYT_RS29710 at 2218.429 kb on + strand, within BPHYT_RS29715 at 2218.874 kb on - strand, within BPHYT_RS29715 at 2219.104 kb on + strand, within BPHYT_RS29715 at 2219.308 kb on + strand, within BPHYT_RS29715 at 2219.308 kb on + strand, within BPHYT_RS29715 at 2219.850 kb on - strand, within BPHYT_RS29715 at 2219.871 kb on + strand, within BPHYT_RS29715 at 2219.953 kb on - strand at 2220.003 kb on + strand at 2220.056 kb on - strand at 2220.107 kb on + strand at 2220.239 kb on + strand at 2220.335 kb on - strand, within BPHYT_RS29720 at 2220.659 kb on + strand, within BPHYT_RS29720 at 2220.869 kb on - strand at 2220.892 kb on - strand at 2220.946 kb on + strand at 2220.990 kb on + strand at 2221.108 kb on - strand, within BPHYT_RS29725 at 2221.108 kb on - strand, within BPHYT_RS29725
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,217,593 + BPHYT_RS29710 0.68 +0.5 2,217,621 - BPHYT_RS29710 0.71 -0.3 2,218,429 + BPHYT_RS29715 0.23 -1.1 2,218,874 - BPHYT_RS29715 0.43 +0.7 2,219,104 + BPHYT_RS29715 0.54 -0.2 2,219,308 + BPHYT_RS29715 0.63 +0.4 2,219,308 + BPHYT_RS29715 0.63 +0.7 2,219,850 - BPHYT_RS29715 0.87 +0.2 2,219,871 + BPHYT_RS29715 0.88 +2.6 2,219,953 - +1.1 2,220,003 + -0.9 2,220,056 - -0.5 2,220,107 + -1.0 2,220,239 + -0.5 2,220,335 - BPHYT_RS29720 0.14 -1.0 2,220,659 + BPHYT_RS29720 0.69 +0.1 2,220,869 - +0.0 2,220,892 - -0.7 2,220,946 + +0.2 2,220,990 + -1.2 2,221,108 - BPHYT_RS29725 0.31 -1.7 2,221,108 - BPHYT_RS29725 0.31 -0.4
Or see this region's nucleotide sequence