Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29365

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29360 and BPHYT_RS29365 are separated by 40 nucleotidesBPHYT_RS29365 and BPHYT_RS29370 are separated by 414 nucleotides BPHYT_RS29360: BPHYT_RS29360 - acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit alpha, at 2,141,192 to 2,142,175 _RS29360 BPHYT_RS29365: BPHYT_RS29365 - ATP-NAD kinase, at 2,142,216 to 2,143,280 _RS29365 BPHYT_RS29370: BPHYT_RS29370 - Fis family transcriptional regulator, at 2,143,695 to 2,145,695 _RS29370 Position (kb) 2142 2143 2144Strain fitness (log2 ratio) -2 -1 0 1at 2141.412 kb on + strand, within BPHYT_RS29360at 2141.908 kb on - strand, within BPHYT_RS29360at 2141.985 kb on + strand, within BPHYT_RS29360at 2141.986 kb on - strand, within BPHYT_RS29360at 2142.042 kb on + strand, within BPHYT_RS29360at 2142.140 kb on + strandat 2142.335 kb on - strand, within BPHYT_RS29365at 2143.395 kb on - strandat 2143.395 kb on - strandat 2143.408 kb on + strandat 2143.408 kb on + strandat 2143.702 kb on - strandat 2144.002 kb on - strand, within BPHYT_RS29370at 2144.049 kb on + strand, within BPHYT_RS29370at 2144.051 kb on + strand, within BPHYT_RS29370

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,141,412 + BPHYT_RS29360 0.22 -0.1
2,141,908 - BPHYT_RS29360 0.73 -0.1
2,141,985 + BPHYT_RS29360 0.81 -1.4
2,141,986 - BPHYT_RS29360 0.81 -0.6
2,142,042 + BPHYT_RS29360 0.86 +1.3
2,142,140 + -0.8
2,142,335 - BPHYT_RS29365 0.11 +0.9
2,143,395 - -2.1
2,143,395 - -1.1
2,143,408 + +0.2
2,143,408 + -0.1
2,143,702 - +1.0
2,144,002 - BPHYT_RS29370 0.15 +0.3
2,144,049 + BPHYT_RS29370 0.18 -0.2
2,144,051 + BPHYT_RS29370 0.18 -2.7

Or see this region's nucleotide sequence