Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29225 and BPHYT_RS29230 are separated by 126 nucleotides BPHYT_RS29230 and BPHYT_RS29235 are separated by 24 nucleotides BPHYT_RS29235 and BPHYT_RS29240 are separated by 175 nucleotides BPHYT_RS29240 and BPHYT_RS29245 overlap by 8 nucleotides
BPHYT_RS29225: BPHYT_RS29225 - diguanylate phosphodiesterase, at 2,112,831 to 2,114,900
_RS29225
BPHYT_RS29230: BPHYT_RS29230 - hypothetical protein, at 2,115,027 to 2,115,833
_RS29230
BPHYT_RS29235: BPHYT_RS29235 - cell shape determination protein CcmA, at 2,115,858 to 2,116,292
_RS29235
BPHYT_RS29240: BPHYT_RS29240 - hypothetical protein, at 2,116,468 to 2,116,866
_RS29240
BPHYT_RS29245: BPHYT_RS29245 - peptidase M23, at 2,116,859 to 2,117,857
_RS29245
Position (kb)
2115
2116
2117 Strain fitness (log2 ratio)
-1
0
1
2 at 2114.999 kb on - strand at 2115.040 kb on + strand at 2115.436 kb on - strand, within BPHYT_RS29230 at 2115.442 kb on + strand, within BPHYT_RS29230 at 2115.442 kb on + strand, within BPHYT_RS29230 at 2115.991 kb on + strand, within BPHYT_RS29235 at 2115.991 kb on + strand, within BPHYT_RS29235 at 2115.992 kb on - strand, within BPHYT_RS29235 at 2116.456 kb on + strand at 2116.894 kb on - strand at 2116.986 kb on + strand, within BPHYT_RS29245 at 2116.987 kb on - strand, within BPHYT_RS29245
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,114,999 - +1.1 2,115,040 + +1.5 2,115,436 - BPHYT_RS29230 0.51 +1.1 2,115,442 + BPHYT_RS29230 0.51 -0.4 2,115,442 + BPHYT_RS29230 0.51 -0.4 2,115,991 + BPHYT_RS29235 0.31 +1.5 2,115,991 + BPHYT_RS29235 0.31 -0.6 2,115,992 - BPHYT_RS29235 0.31 +2.6 2,116,456 + -0.8 2,116,894 - -0.6 2,116,986 + BPHYT_RS29245 0.13 +0.3 2,116,987 - BPHYT_RS29245 0.13 -0.7
Or see this region's nucleotide sequence