Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29215

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29210 and BPHYT_RS29215 are separated by 472 nucleotidesBPHYT_RS29215 and BPHYT_RS29220 are separated by 86 nucleotides BPHYT_RS29210: BPHYT_RS29210 - rhodanese, at 2,109,729 to 2,110,169 _RS29210 BPHYT_RS29215: BPHYT_RS29215 - porin, at 2,110,642 to 2,111,817 _RS29215 BPHYT_RS29220: BPHYT_RS29220 - hypothetical protein, at 2,111,904 to 2,112,644 _RS29220 Position (kb) 2110 2111 2112Strain fitness (log2 ratio) -1 0 1 2at 2110.169 kb on + strandat 2110.170 kb on - strandat 2110.170 kb on - strandat 2110.170 kb on - strandat 2110.617 kb on - strandat 2111.176 kb on + strand, within BPHYT_RS29215at 2111.177 kb on - strand, within BPHYT_RS29215at 2111.521 kb on + strand, within BPHYT_RS29215at 2111.540 kb on - strand, within BPHYT_RS29215at 2111.540 kb on - strand, within BPHYT_RS29215at 2111.547 kb on + strand, within BPHYT_RS29215at 2111.556 kb on + strand, within BPHYT_RS29215at 2111.707 kb on + strandat 2111.732 kb on - strandat 2111.732 kb on - strandat 2111.742 kb on + strandat 2112.160 kb on + strand, within BPHYT_RS29220at 2112.207 kb on - strand, within BPHYT_RS29220at 2112.208 kb on + strand, within BPHYT_RS29220at 2112.252 kb on - strand, within BPHYT_RS29220at 2112.458 kb on - strand, within BPHYT_RS29220at 2112.560 kb on - strand, within BPHYT_RS29220at 2112.578 kb on - strandat 2112.772 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,110,169 + +1.1
2,110,170 - +0.1
2,110,170 - +0.4
2,110,170 - +0.4
2,110,617 - -1.0
2,111,176 + BPHYT_RS29215 0.45 +0.9
2,111,177 - BPHYT_RS29215 0.45 +1.9
2,111,521 + BPHYT_RS29215 0.75 -0.1
2,111,540 - BPHYT_RS29215 0.76 +2.1
2,111,540 - BPHYT_RS29215 0.76 +0.3
2,111,547 + BPHYT_RS29215 0.77 +0.5
2,111,556 + BPHYT_RS29215 0.78 +1.1
2,111,707 + -1.2
2,111,732 - +1.3
2,111,732 - +1.0
2,111,742 + -0.2
2,112,160 + BPHYT_RS29220 0.35 +1.6
2,112,207 - BPHYT_RS29220 0.41 +0.6
2,112,208 + BPHYT_RS29220 0.41 +0.3
2,112,252 - BPHYT_RS29220 0.47 -0.7
2,112,458 - BPHYT_RS29220 0.75 +1.3
2,112,560 - BPHYT_RS29220 0.89 -1.3
2,112,578 - +1.2
2,112,772 - -0.6

Or see this region's nucleotide sequence