Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28730 and BPHYT_RS28735 are separated by 160 nucleotides BPHYT_RS28735 and BPHYT_RS28740 are separated by 29 nucleotides BPHYT_RS28740 and BPHYT_RS28745 are separated by 13 nucleotides BPHYT_RS28745 and BPHYT_RS28750 are separated by 65 nucleotides
BPHYT_RS28730: BPHYT_RS28730 - galactarate dehydratase, at 1,999,039 to 2,000,550
_RS28730
BPHYT_RS28735: BPHYT_RS28735 - hypothetical protein, at 2,000,711 to 2,001,406
_RS28735
BPHYT_RS28740: BPHYT_RS28740 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,001,436 to 2,002,326
_RS28740
BPHYT_RS28745: BPHYT_RS28745 - 2-keto-4-pentenoate hydratase, at 2,002,340 to 2,003,149
_RS28745
BPHYT_RS28750: BPHYT_RS28750 - IclR family transcriptional regulator, at 2,003,215 to 2,003,499
_RS28750
Position (kb)
2001
2002
2003 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2000.817 kb on - strand, within BPHYT_RS28735 at 2000.878 kb on + strand, within BPHYT_RS28735 at 2000.879 kb on - strand, within BPHYT_RS28735 at 2001.073 kb on - strand, within BPHYT_RS28735 at 2001.077 kb on - strand, within BPHYT_RS28735 at 2001.077 kb on - strand, within BPHYT_RS28735 at 2001.406 kb on + strand at 2001.438 kb on - strand at 2001.480 kb on + strand at 2001.489 kb on - strand at 2001.609 kb on + strand, within BPHYT_RS28740 at 2001.651 kb on - strand, within BPHYT_RS28740 at 2001.810 kb on + strand, within BPHYT_RS28740 at 2002.428 kb on + strand, within BPHYT_RS28745 at 2002.778 kb on - strand, within BPHYT_RS28745 at 2002.782 kb on - strand, within BPHYT_RS28745 at 2002.843 kb on + strand, within BPHYT_RS28745
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,000,817 - BPHYT_RS28735 0.15 +1.6 2,000,878 + BPHYT_RS28735 0.24 +0.0 2,000,879 - BPHYT_RS28735 0.24 -0.3 2,001,073 - BPHYT_RS28735 0.52 -0.1 2,001,077 - BPHYT_RS28735 0.53 -2.2 2,001,077 - BPHYT_RS28735 0.53 -0.7 2,001,406 + -0.1 2,001,438 - +0.3 2,001,480 + -0.4 2,001,489 - -1.2 2,001,609 + BPHYT_RS28740 0.19 -0.2 2,001,651 - BPHYT_RS28740 0.24 +1.6 2,001,810 + BPHYT_RS28740 0.42 +2.3 2,002,428 + BPHYT_RS28745 0.11 -0.1 2,002,778 - BPHYT_RS28745 0.54 -1.1 2,002,782 - BPHYT_RS28745 0.55 -2.3 2,002,843 + BPHYT_RS28745 0.62 +0.3
Or see this region's nucleotide sequence