Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27370

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27355 and BPHYT_RS27360 overlap by 4 nucleotidesBPHYT_RS27360 and BPHYT_RS27365 are separated by 1 nucleotidesBPHYT_RS27365 and BPHYT_RS27370 overlap by 1 nucleotidesBPHYT_RS27370 and BPHYT_RS27375 overlap by 1 nucleotidesBPHYT_RS27375 and BPHYT_RS27380 are separated by 8 nucleotides BPHYT_RS27355: BPHYT_RS27355 - 2-dehydropantoate 2-reductase, at 1,712,694 to 1,713,665 _RS27355 BPHYT_RS27360: BPHYT_RS27360 - glyoxalase, at 1,713,662 to 1,714,054 _RS27360 BPHYT_RS27365: BPHYT_RS27365 - acyl dehydratase, at 1,714,056 to 1,714,514 _RS27365 BPHYT_RS27370: BPHYT_RS27370 - CoA transferase, at 1,714,514 to 1,715,734 _RS27370 BPHYT_RS27375: BPHYT_RS27375 - shikimate 5-dehydrogenase, at 1,715,734 to 1,716,567 _RS27375 BPHYT_RS27380: BPHYT_RS27380 - 3-dehydroquinate dehydratase, at 1,716,576 to 1,716,683 _RS27380 Position (kb) 1714 1715 1716Strain fitness (log2 ratio) -1 0 1 2at 1713.680 kb on + strandat 1714.120 kb on - strand, within BPHYT_RS27365at 1715.028 kb on - strand, within BPHYT_RS27370at 1715.118 kb on - strand, within BPHYT_RS27370at 1715.145 kb on - strand, within BPHYT_RS27370at 1715.220 kb on - strand, within BPHYT_RS27370at 1715.856 kb on - strand, within BPHYT_RS27375at 1716.265 kb on + strand, within BPHYT_RS27375

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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1,713,680 + -0.4
1,714,120 - BPHYT_RS27365 0.14 +0.4
1,715,028 - BPHYT_RS27370 0.42 -1.4
1,715,118 - BPHYT_RS27370 0.49 +0.0
1,715,145 - BPHYT_RS27370 0.52 +2.3
1,715,220 - BPHYT_RS27370 0.58 -1.2
1,715,856 - BPHYT_RS27375 0.15 -0.5
1,716,265 + BPHYT_RS27375 0.64 -0.2

Or see this region's nucleotide sequence