Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26745 and BPHYT_RS26750 are separated by 4 nucleotides BPHYT_RS26750 and BPHYT_RS26755 are separated by 53 nucleotides BPHYT_RS26755 and BPHYT_RS26760 are separated by 236 nucleotides
BPHYT_RS26745: BPHYT_RS26745 - 2-dehydropantoate 2-reductase, at 1,570,369 to 1,571,352
_RS26745
BPHYT_RS26750: BPHYT_RS26750 - aldolase, at 1,571,357 to 1,572,148
_RS26750
BPHYT_RS26755: BPHYT_RS26755 - aromatic amino acid aminotransferase, at 1,572,202 to 1,573,401
_RS26755
BPHYT_RS26760: BPHYT_RS26760 - LysR family transcriptional regulator, at 1,573,638 to 1,574,579
_RS26760
Position (kb)
1572
1573
1574 Strain fitness (log2 ratio)
-2
-1
0
1 at 1572.005 kb on - strand, within BPHYT_RS26750 at 1572.015 kb on + strand, within BPHYT_RS26750 at 1572.243 kb on - strand at 1572.243 kb on - strand at 1572.243 kb on - strand at 1572.300 kb on - strand at 1572.300 kb on - strand at 1572.380 kb on + strand, within BPHYT_RS26755 at 1572.957 kb on - strand, within BPHYT_RS26755 at 1572.962 kb on + strand, within BPHYT_RS26755 at 1573.034 kb on - strand, within BPHYT_RS26755 at 1573.053 kb on - strand, within BPHYT_RS26755 at 1573.156 kb on + strand, within BPHYT_RS26755 at 1573.157 kb on - strand, within BPHYT_RS26755 at 1573.181 kb on + strand, within BPHYT_RS26755 at 1573.529 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 1,572,005 - BPHYT_RS26750 0.82 -0.1 1,572,015 + BPHYT_RS26750 0.83 +0.7 1,572,243 - -1.6 1,572,243 - +0.1 1,572,243 - +0.5 1,572,300 - -1.0 1,572,300 - -0.6 1,572,380 + BPHYT_RS26755 0.15 +1.6 1,572,957 - BPHYT_RS26755 0.63 -0.9 1,572,962 + BPHYT_RS26755 0.63 +0.9 1,573,034 - BPHYT_RS26755 0.69 -0.7 1,573,053 - BPHYT_RS26755 0.71 +0.7 1,573,156 + BPHYT_RS26755 0.80 -0.1 1,573,157 - BPHYT_RS26755 0.80 +0.3 1,573,181 + BPHYT_RS26755 0.82 -2.5 1,573,529 + +1.2
Or see this region's nucleotide sequence