Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24995 and BPHYT_RS25000 are separated by 471 nucleotides BPHYT_RS25000 and BPHYT_RS25005 are separated by 38 nucleotides BPHYT_RS25005 and BPHYT_RS25010 are separated by 194 nucleotides
BPHYT_RS24995: BPHYT_RS24995 - hypothetical protein, at 1,176,974 to 1,178,122
_RS24995
BPHYT_RS25000: BPHYT_RS25000 - MFS transporter, at 1,178,594 to 1,179,838
_RS25000
BPHYT_RS25005: BPHYT_RS25005 - FMN reductase, at 1,179,877 to 1,180,422
_RS25005
BPHYT_RS25010: BPHYT_RS25010 - N5,N10-methylene tetrahydromethanopterin reductase, at 1,180,617 to 1,182,008
_RS25010
Position (kb) 1178
1179
1180 Strain fitness (log2 ratio) -2
-1
0
1 at 1177.709 kb on - strand, within BPHYT_RS24995 at 1178.581 kb on - strand at 1178.657 kb on - strand at 1178.657 kb on - strand at 1179.309 kb on + strand, within BPHYT_RS25000 at 1179.310 kb on - strand, within BPHYT_RS25000 at 1179.310 kb on - strand, within BPHYT_RS25000 at 1180.002 kb on + strand, within BPHYT_RS25005 at 1180.003 kb on - strand, within BPHYT_RS25005 at 1180.747 kb on + strand at 1180.818 kb on - strand, within BPHYT_RS25010
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 1,177,709 - BPHYT_RS24995 0.64 -2.4 1,178,581 - +0.4 1,178,657 - -0.8 1,178,657 - +0.0 1,179,309 + BPHYT_RS25000 0.57 +1.5 1,179,310 - BPHYT_RS25000 0.58 +0.2 1,179,310 - BPHYT_RS25000 0.58 +1.0 1,180,002 + BPHYT_RS25005 0.23 +1.0 1,180,003 - BPHYT_RS25005 0.23 +0.6 1,180,747 + +0.6 1,180,818 - BPHYT_RS25010 0.14 -0.6
Or see this region's nucleotide sequence