Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23675 and BPHYT_RS23680 are separated by 71 nucleotides BPHYT_RS23680 and BPHYT_RS23685 are separated by 139 nucleotides BPHYT_RS23685 and BPHYT_RS23690 are separated by 96 nucleotides BPHYT_RS23690 and BPHYT_RS23695 are separated by 302 nucleotides
BPHYT_RS23675: BPHYT_RS23675 - lysine transporter LysE, at 881,109 to 881,762
_RS23675
BPHYT_RS23680: BPHYT_RS23680 - Fe-S protein, at 881,834 to 882,007
_RS23680
BPHYT_RS23685: BPHYT_RS23685 - membrane protein, at 882,147 to 882,467
_RS23685
BPHYT_RS23690: BPHYT_RS23690 - hypothetical protein, at 882,564 to 883,073
_RS23690
BPHYT_RS23695: BPHYT_RS23695 - peptidase S53, at 883,376 to 884,935
_RS23695
Position (kb)
882
883
884 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 882.029 kb on - strand at 882.034 kb on - strand at 882.087 kb on + strand at 882.097 kb on - strand at 882.111 kb on + strand at 882.321 kb on + strand, within BPHYT_RS23685 at 882.322 kb on - strand, within BPHYT_RS23685 at 882.343 kb on - strand, within BPHYT_RS23685 at 882.566 kb on - strand at 882.566 kb on - strand at 882.629 kb on - strand, within BPHYT_RS23690 at 882.649 kb on + strand, within BPHYT_RS23690 at 882.680 kb on - strand, within BPHYT_RS23690 at 882.779 kb on - strand, within BPHYT_RS23690 at 882.839 kb on - strand, within BPHYT_RS23690 at 882.839 kb on - strand, within BPHYT_RS23690 at 882.901 kb on + strand, within BPHYT_RS23690 at 882.901 kb on + strand, within BPHYT_RS23690 at 882.901 kb on + strand, within BPHYT_RS23690 at 883.007 kb on + strand, within BPHYT_RS23690 at 883.057 kb on + strand at 883.058 kb on - strand at 883.058 kb on - strand at 883.126 kb on + strand at 883.127 kb on - strand at 883.470 kb on + strand at 883.470 kb on + strand at 883.471 kb on - strand at 883.935 kb on - strand, within BPHYT_RS23695 at 883.997 kb on + strand, within BPHYT_RS23695
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 882,029 - -2.5 882,034 - -0.7 882,087 + -0.3 882,097 - +0.6 882,111 + +1.1 882,321 + BPHYT_RS23685 0.54 +0.7 882,322 - BPHYT_RS23685 0.55 +0.1 882,343 - BPHYT_RS23685 0.61 +1.1 882,566 - -0.3 882,566 - -0.8 882,629 - BPHYT_RS23690 0.13 -0.2 882,649 + BPHYT_RS23690 0.17 -1.3 882,680 - BPHYT_RS23690 0.23 -0.5 882,779 - BPHYT_RS23690 0.42 +2.7 882,839 - BPHYT_RS23690 0.54 +2.3 882,839 - BPHYT_RS23690 0.54 +0.5 882,901 + BPHYT_RS23690 0.66 +0.2 882,901 + BPHYT_RS23690 0.66 +0.1 882,901 + BPHYT_RS23690 0.66 -1.9 883,007 + BPHYT_RS23690 0.87 -0.2 883,057 + -0.2 883,058 - +0.7 883,058 - +0.7 883,126 + +0.1 883,127 - -0.4 883,470 + -1.9 883,470 + +0.3 883,471 - -0.1 883,935 - BPHYT_RS23695 0.36 +0.5 883,997 + BPHYT_RS23695 0.40 +0.8
Or see this region's nucleotide sequence