Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23235 and BPHYT_RS23240 are separated by 350 nucleotides BPHYT_RS23240 and BPHYT_RS23245 are separated by 203 nucleotides BPHYT_RS23245 and BPHYT_RS23250 are separated by 127 nucleotides
BPHYT_RS23235: BPHYT_RS23235 - hypothetical protein, at 777,889 to 778,272
_RS23235
BPHYT_RS23240: BPHYT_RS23240 - DNA-binding protein, at 778,623 to 778,901
_RS23240
BPHYT_RS23245: BPHYT_RS23245 - phospho-2-dehydro-3-deoxyheptonate aldolase, at 779,105 to 780,223
_RS23245
BPHYT_RS23250: BPHYT_RS23250 - hypothetical protein, at 780,351 to 781,172
_RS23250
Position (kb) 779
780
781 Strain fitness (log2 ratio) -2
-1
0
1
2 at 778.296 kb on + strand at 778.301 kb on - strand at 778.323 kb on - strand at 778.345 kb on + strand at 778.431 kb on + strand at 778.431 kb on + strand at 778.467 kb on - strand at 778.514 kb on + strand at 778.514 kb on + strand at 778.515 kb on - strand at 778.523 kb on + strand at 778.559 kb on + strand at 778.605 kb on + strand at 778.611 kb on - strand at 778.622 kb on + strand at 778.623 kb on - strand at 779.042 kb on - strand at 779.710 kb on - strand, within BPHYT_RS23245 at 779.710 kb on - strand, within BPHYT_RS23245 at 779.844 kb on + strand, within BPHYT_RS23245 at 779.844 kb on + strand, within BPHYT_RS23245 at 779.845 kb on - strand, within BPHYT_RS23245 at 779.883 kb on + strand, within BPHYT_RS23245 at 779.916 kb on + strand, within BPHYT_RS23245 at 779.917 kb on - strand, within BPHYT_RS23245 at 780.421 kb on + strand at 780.422 kb on - strand at 780.585 kb on + strand, within BPHYT_RS23250 at 780.711 kb on + strand, within BPHYT_RS23250 at 781.209 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 778,296 + +0.6 778,301 - -0.8 778,323 - -1.9 778,345 + +0.1 778,431 + -1.1 778,431 + -0.2 778,467 - -0.0 778,514 + -0.7 778,514 + -0.1 778,515 - +0.9 778,523 + -1.2 778,559 + -0.8 778,605 + -0.6 778,611 - -1.5 778,622 + +0.4 778,623 - -0.5 779,042 - +1.8 779,710 - BPHYT_RS23245 0.54 -0.1 779,710 - BPHYT_RS23245 0.54 +0.6 779,844 + BPHYT_RS23245 0.66 -0.2 779,844 + BPHYT_RS23245 0.66 +1.7 779,845 - BPHYT_RS23245 0.66 -1.5 779,883 + BPHYT_RS23245 0.70 -2.0 779,916 + BPHYT_RS23245 0.72 -1.3 779,917 - BPHYT_RS23245 0.73 -1.2 780,421 + -0.0 780,422 - +0.9 780,585 + BPHYT_RS23250 0.28 -2.2 780,711 + BPHYT_RS23250 0.44 +1.6 781,209 - +0.7
Or see this region's nucleotide sequence