Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22930 and BPHYT_RS22935 are separated by 26 nucleotides BPHYT_RS22935 and BPHYT_RS22940 overlap by 4 nucleotides BPHYT_RS22940 and BPHYT_RS22945 are separated by 1 nucleotides BPHYT_RS22945 and BPHYT_RS22950 are separated by 53 nucleotides
BPHYT_RS22930: BPHYT_RS22930 - type II secretion system protein, at 703,406 to 704,362
_RS22930
BPHYT_RS22935: BPHYT_RS22935 - fatty acid hydroxylase, at 704,389 to 705,255
_RS22935
BPHYT_RS22940: BPHYT_RS22940 - pilus assembly protein TadE, at 705,252 to 705,782
_RS22940
BPHYT_RS22945: BPHYT_RS22945 - pilus assembly protein TadE, at 705,784 to 706,413
_RS22945
BPHYT_RS22950: BPHYT_RS22950 - von Willebrand factor A, at 706,467 to 707,822
_RS22950
Position (kb) 705
706 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 704.374 kb on - strand at 704.563 kb on + strand, within BPHYT_RS22935 at 704.563 kb on + strand, within BPHYT_RS22935 at 704.742 kb on + strand, within BPHYT_RS22935 at 704.743 kb on - strand, within BPHYT_RS22935 at 704.743 kb on - strand, within BPHYT_RS22935 at 704.743 kb on - strand, within BPHYT_RS22935 at 704.857 kb on + strand, within BPHYT_RS22935 at 704.936 kb on - strand, within BPHYT_RS22935 at 705.514 kb on - strand, within BPHYT_RS22940 at 705.630 kb on + strand, within BPHYT_RS22940 at 705.630 kb on + strand, within BPHYT_RS22940 at 705.887 kb on - strand, within BPHYT_RS22945 at 706.051 kb on + strand, within BPHYT_RS22945 at 706.052 kb on - strand, within BPHYT_RS22945 at 706.322 kb on + strand, within BPHYT_RS22945 at 706.393 kb on + strand at 706.599 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 704,374 - -0.3 704,563 + BPHYT_RS22935 0.20 -0.9 704,563 + BPHYT_RS22935 0.20 +0.4 704,742 + BPHYT_RS22935 0.41 -0.8 704,743 - BPHYT_RS22935 0.41 -2.2 704,743 - BPHYT_RS22935 0.41 +0.3 704,743 - BPHYT_RS22935 0.41 -3.3 704,857 + BPHYT_RS22935 0.54 -1.6 704,936 - BPHYT_RS22935 0.63 -1.0 705,514 - BPHYT_RS22940 0.49 +2.1 705,630 + BPHYT_RS22940 0.71 +0.7 705,630 + BPHYT_RS22940 0.71 +1.9 705,887 - BPHYT_RS22945 0.16 -0.1 706,051 + BPHYT_RS22945 0.42 +0.0 706,052 - BPHYT_RS22945 0.43 -0.2 706,322 + BPHYT_RS22945 0.85 +1.6 706,393 + -0.4 706,599 - -1.3
Or see this region's nucleotide sequence