Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22925 and BPHYT_RS22930 are separated by 43 nucleotides BPHYT_RS22930 and BPHYT_RS22935 are separated by 26 nucleotides BPHYT_RS22935 and BPHYT_RS22940 overlap by 4 nucleotides
BPHYT_RS22925: BPHYT_RS22925 - pilus assembly protein TadB, at 702,385 to 703,362
_RS22925
BPHYT_RS22930: BPHYT_RS22930 - type II secretion system protein, at 703,406 to 704,362
_RS22930
BPHYT_RS22935: BPHYT_RS22935 - fatty acid hydroxylase, at 704,389 to 705,255
_RS22935
BPHYT_RS22940: BPHYT_RS22940 - pilus assembly protein TadE, at 705,252 to 705,782
_RS22940
Position (kb) 703
704
705 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 702.688 kb on + strand, within BPHYT_RS22925 at 702.689 kb on - strand, within BPHYT_RS22925 at 702.689 kb on - strand, within BPHYT_RS22925 at 702.930 kb on - strand, within BPHYT_RS22925 at 703.835 kb on + strand, within BPHYT_RS22930 at 704.175 kb on - strand, within BPHYT_RS22930 at 704.374 kb on - strand at 704.563 kb on + strand, within BPHYT_RS22935 at 704.563 kb on + strand, within BPHYT_RS22935 at 704.742 kb on + strand, within BPHYT_RS22935 at 704.743 kb on - strand, within BPHYT_RS22935 at 704.743 kb on - strand, within BPHYT_RS22935 at 704.743 kb on - strand, within BPHYT_RS22935 at 704.857 kb on + strand, within BPHYT_RS22935 at 704.936 kb on - strand, within BPHYT_RS22935
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 702,688 + BPHYT_RS22925 0.31 -1.0 702,689 - BPHYT_RS22925 0.31 -0.9 702,689 - BPHYT_RS22925 0.31 -0.6 702,930 - BPHYT_RS22925 0.56 -0.3 703,835 + BPHYT_RS22930 0.45 -1.9 704,175 - BPHYT_RS22930 0.80 +1.7 704,374 - -0.3 704,563 + BPHYT_RS22935 0.20 -0.9 704,563 + BPHYT_RS22935 0.20 +0.4 704,742 + BPHYT_RS22935 0.41 -0.8 704,743 - BPHYT_RS22935 0.41 -2.2 704,743 - BPHYT_RS22935 0.41 +0.3 704,743 - BPHYT_RS22935 0.41 -3.3 704,857 + BPHYT_RS22935 0.54 -1.6 704,936 - BPHYT_RS22935 0.63 -1.0
Or see this region's nucleotide sequence