Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22415 and BPHYT_RS22420 are separated by 328 nucleotides BPHYT_RS22420 and BPHYT_RS22425 are separated by 32 nucleotides BPHYT_RS22425 and BPHYT_RS22430 are separated by 221 nucleotides
BPHYT_RS22415: BPHYT_RS22415 - nitrite reductase, at 582,910 to 584,700
_RS22415
BPHYT_RS22420: BPHYT_RS22420 - membrane protein, at 585,029 to 585,217
_RS22420
BPHYT_RS22425: BPHYT_RS22425 - GntR family transcriptional regulator, at 585,250 to 586,665
_RS22425
BPHYT_RS22430: BPHYT_RS22430 - succinate-semialdehyde dehydrogenase, at 586,887 to 588,347
_RS22430
Position (kb) 585
586
587 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 584.468 kb on + strand, within BPHYT_RS22415 at 584.469 kb on - strand, within BPHYT_RS22415 at 584.690 kb on + strand at 584.690 kb on + strand at 585.148 kb on - strand, within BPHYT_RS22420 at 585.339 kb on + strand at 585.465 kb on - strand, within BPHYT_RS22425 at 585.465 kb on - strand, within BPHYT_RS22425 at 585.507 kb on - strand, within BPHYT_RS22425 at 585.507 kb on - strand, within BPHYT_RS22425 at 585.582 kb on - strand, within BPHYT_RS22425 at 585.942 kb on + strand, within BPHYT_RS22425 at 586.188 kb on - strand, within BPHYT_RS22425 at 586.198 kb on + strand, within BPHYT_RS22425 at 586.759 kb on - strand at 586.811 kb on + strand at 586.910 kb on - strand at 586.925 kb on - strand at 586.925 kb on - strand at 587.083 kb on + strand, within BPHYT_RS22430 at 587.084 kb on - strand, within BPHYT_RS22430 at 587.084 kb on - strand, within BPHYT_RS22430 at 587.084 kb on - strand, within BPHYT_RS22430 at 587.452 kb on + strand, within BPHYT_RS22430 at 587.453 kb on - strand, within BPHYT_RS22430 at 587.453 kb on - strand, within BPHYT_RS22430
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 584,468 + BPHYT_RS22415 0.87 -0.4 584,469 - BPHYT_RS22415 0.87 +0.2 584,690 + +0.7 584,690 + -1.1 585,148 - BPHYT_RS22420 0.63 +0.3 585,339 + -0.3 585,465 - BPHYT_RS22425 0.15 +0.8 585,465 - BPHYT_RS22425 0.15 +0.3 585,507 - BPHYT_RS22425 0.18 -1.0 585,507 - BPHYT_RS22425 0.18 +0.6 585,582 - BPHYT_RS22425 0.23 +0.8 585,942 + BPHYT_RS22425 0.49 +1.4 586,188 - BPHYT_RS22425 0.66 +4.2 586,198 + BPHYT_RS22425 0.67 +0.2 586,759 - +1.2 586,811 + -0.9 586,910 - +1.4 586,925 - +0.4 586,925 - -1.2 587,083 + BPHYT_RS22430 0.13 +0.1 587,084 - BPHYT_RS22430 0.13 -0.0 587,084 - BPHYT_RS22430 0.13 -0.4 587,084 - BPHYT_RS22430 0.13 -0.4 587,452 + BPHYT_RS22430 0.39 -0.1 587,453 - BPHYT_RS22430 0.39 +0.2 587,453 - BPHYT_RS22430 0.39 -0.0
Or see this region's nucleotide sequence