Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22250 and BPHYT_RS22255 are separated by 5 nucleotides BPHYT_RS22255 and BPHYT_RS22260 are separated by 58 nucleotides BPHYT_RS22260 and BPHYT_RS22265 are separated by 106 nucleotides BPHYT_RS22265 and BPHYT_RS22270 are separated by 10 nucleotides
BPHYT_RS22250: BPHYT_RS22250 - D-amino acid dehydrogenase, at 549,665 to 550,909
_RS22250
BPHYT_RS22255: BPHYT_RS22255 - hypothetical protein, at 550,915 to 551,259
_RS22255
BPHYT_RS22260: BPHYT_RS22260 - hypothetical protein, at 551,318 to 551,608
_RS22260
BPHYT_RS22265: BPHYT_RS22265 - hypothetical protein, at 551,715 to 552,506
_RS22265
BPHYT_RS22270: BPHYT_RS22270 - membrane protein, at 552,517 to 555,135
_RS22270
Position (kb) 551
552
553 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 550.917 kb on - strand at 550.917 kb on - strand at 550.919 kb on - strand at 550.919 kb on - strand at 551.070 kb on - strand, within BPHYT_RS22255 at 551.267 kb on + strand at 551.345 kb on + strand at 551.516 kb on - strand, within BPHYT_RS22260 at 551.606 kb on + strand at 551.607 kb on - strand at 551.623 kb on + strand at 551.715 kb on - strand at 551.768 kb on - strand at 551.768 kb on - strand at 551.940 kb on + strand, within BPHYT_RS22265 at 551.976 kb on - strand, within BPHYT_RS22265 at 552.516 kb on + strand at 552.516 kb on + strand at 552.517 kb on - strand at 552.517 kb on - strand at 552.526 kb on + strand at 552.530 kb on + strand at 552.643 kb on + strand at 552.643 kb on + strand at 552.644 kb on - strand at 552.673 kb on + strand at 553.120 kb on + strand, within BPHYT_RS22270 at 553.300 kb on + strand, within BPHYT_RS22270 at 553.302 kb on + strand, within BPHYT_RS22270 at 553.303 kb on - strand, within BPHYT_RS22270
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 550,917 - +0.3 550,917 - -0.4 550,919 - -0.4 550,919 - +1.6 551,070 - BPHYT_RS22255 0.45 +0.1 551,267 + -0.8 551,345 + -0.1 551,516 - BPHYT_RS22260 0.68 -0.7 551,606 + +1.2 551,607 - -0.3 551,623 + +0.2 551,715 - -1.9 551,768 - +0.2 551,768 - -1.2 551,940 + BPHYT_RS22265 0.28 -1.0 551,976 - BPHYT_RS22265 0.33 -1.0 552,516 + -1.1 552,516 + +2.8 552,517 - +1.3 552,517 - -1.3 552,526 + +0.2 552,530 + -0.4 552,643 + -1.0 552,643 + -0.3 552,644 - +1.2 552,673 + +1.3 553,120 + BPHYT_RS22270 0.23 +0.3 553,300 + BPHYT_RS22270 0.30 -0.0 553,302 + BPHYT_RS22270 0.30 +0.8 553,303 - BPHYT_RS22270 0.30 +0.5
Or see this region's nucleotide sequence