Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22265

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22250 and BPHYT_RS22255 are separated by 5 nucleotidesBPHYT_RS22255 and BPHYT_RS22260 are separated by 58 nucleotidesBPHYT_RS22260 and BPHYT_RS22265 are separated by 106 nucleotidesBPHYT_RS22265 and BPHYT_RS22270 are separated by 10 nucleotides BPHYT_RS22250: BPHYT_RS22250 - D-amino acid dehydrogenase, at 549,665 to 550,909 _RS22250 BPHYT_RS22255: BPHYT_RS22255 - hypothetical protein, at 550,915 to 551,259 _RS22255 BPHYT_RS22260: BPHYT_RS22260 - hypothetical protein, at 551,318 to 551,608 _RS22260 BPHYT_RS22265: BPHYT_RS22265 - hypothetical protein, at 551,715 to 552,506 _RS22265 BPHYT_RS22270: BPHYT_RS22270 - membrane protein, at 552,517 to 555,135 _RS22270 Position (kb) 551 552 553Strain fitness (log2 ratio) -2 -1 0 1 2 3at 550.917 kb on - strandat 550.917 kb on - strandat 550.919 kb on - strandat 550.919 kb on - strandat 551.070 kb on - strand, within BPHYT_RS22255at 551.267 kb on + strandat 551.345 kb on + strandat 551.516 kb on - strand, within BPHYT_RS22260at 551.606 kb on + strandat 551.607 kb on - strandat 551.623 kb on + strandat 551.715 kb on - strandat 551.768 kb on - strandat 551.768 kb on - strandat 551.940 kb on + strand, within BPHYT_RS22265at 551.976 kb on - strand, within BPHYT_RS22265at 552.516 kb on + strandat 552.516 kb on + strandat 552.517 kb on - strandat 552.517 kb on - strandat 552.526 kb on + strandat 552.530 kb on + strandat 552.643 kb on + strandat 552.643 kb on + strandat 552.644 kb on - strandat 552.673 kb on + strandat 553.120 kb on + strand, within BPHYT_RS22270at 553.300 kb on + strand, within BPHYT_RS22270at 553.302 kb on + strand, within BPHYT_RS22270at 553.303 kb on - strand, within BPHYT_RS22270

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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550,917 - +0.3
550,917 - -0.4
550,919 - -0.4
550,919 - +1.6
551,070 - BPHYT_RS22255 0.45 +0.1
551,267 + -0.8
551,345 + -0.1
551,516 - BPHYT_RS22260 0.68 -0.7
551,606 + +1.2
551,607 - -0.3
551,623 + +0.2
551,715 - -1.9
551,768 - +0.2
551,768 - -1.2
551,940 + BPHYT_RS22265 0.28 -1.0
551,976 - BPHYT_RS22265 0.33 -1.0
552,516 + -1.1
552,516 + +2.8
552,517 - +1.3
552,517 - -1.3
552,526 + +0.2
552,530 + -0.4
552,643 + -1.0
552,643 + -0.3
552,644 - +1.2
552,673 + +1.3
553,120 + BPHYT_RS22270 0.23 +0.3
553,300 + BPHYT_RS22270 0.30 -0.0
553,302 + BPHYT_RS22270 0.30 +0.8
553,303 - BPHYT_RS22270 0.30 +0.5

Or see this region's nucleotide sequence