Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22035 and BPHYT_RS22040 are separated by 1 nucleotides BPHYT_RS22040 and BPHYT_RS22045 are separated by 3 nucleotides BPHYT_RS22045 and BPHYT_RS22050 are separated by 17 nucleotides
BPHYT_RS22035: BPHYT_RS22035 - peptide ABC transporter ATP-binding protein, at 498,983 to 499,981
_RS22035
BPHYT_RS22040: BPHYT_RS22040 - peptide ABC transporter permease, at 499,983 to 500,897
_RS22040
BPHYT_RS22045: BPHYT_RS22045 - peptide ABC transporter permease, at 500,901 to 501,839
_RS22045
BPHYT_RS22050: BPHYT_RS22050 - peptide ABC transporter substrate-binding protein, at 501,857 to 503,473
_RS22050
Position (kb)
500
501
502 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 500.041 kb on + strand at 500.219 kb on - strand, within BPHYT_RS22040 at 500.446 kb on + strand, within BPHYT_RS22040 at 500.447 kb on - strand, within BPHYT_RS22040 at 500.447 kb on - strand, within BPHYT_RS22040 at 500.459 kb on - strand, within BPHYT_RS22040 at 500.933 kb on - strand at 501.652 kb on + strand, within BPHYT_RS22045 at 501.683 kb on - strand, within BPHYT_RS22045 at 501.683 kb on - strand, within BPHYT_RS22045 at 501.828 kb on - strand at 501.873 kb on + strand at 501.873 kb on + strand at 501.874 kb on - strand at 501.874 kb on - strand at 501.903 kb on + strand at 501.932 kb on - strand at 501.982 kb on - strand at 502.090 kb on - strand, within BPHYT_RS22050 at 502.153 kb on - strand, within BPHYT_RS22050 at 502.221 kb on + strand, within BPHYT_RS22050 at 502.576 kb on + strand, within BPHYT_RS22050 at 502.587 kb on + strand, within BPHYT_RS22050 at 502.587 kb on + strand, within BPHYT_RS22050 at 502.588 kb on - strand, within BPHYT_RS22050 at 502.590 kb on + strand, within BPHYT_RS22050 at 502.594 kb on - strand, within BPHYT_RS22050 at 502.836 kb on + strand, within BPHYT_RS22050
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 500,041 + -2.7 500,219 - BPHYT_RS22040 0.26 +0.6 500,446 + BPHYT_RS22040 0.51 -2.1 500,447 - BPHYT_RS22040 0.51 -0.8 500,447 - BPHYT_RS22040 0.51 +0.6 500,459 - BPHYT_RS22040 0.52 -0.2 500,933 - -2.4 501,652 + BPHYT_RS22045 0.80 -0.0 501,683 - BPHYT_RS22045 0.83 +2.3 501,683 - BPHYT_RS22045 0.83 -1.2 501,828 - -0.8 501,873 + +0.2 501,873 + +0.7 501,874 - -1.3 501,874 - -2.0 501,903 + -3.4 501,932 - -1.7 501,982 - -0.6 502,090 - BPHYT_RS22050 0.14 -0.6 502,153 - BPHYT_RS22050 0.18 +0.6 502,221 + BPHYT_RS22050 0.23 -0.1 502,576 + BPHYT_RS22050 0.44 -1.2 502,587 + BPHYT_RS22050 0.45 -0.8 502,587 + BPHYT_RS22050 0.45 -0.9 502,588 - BPHYT_RS22050 0.45 -2.5 502,590 + BPHYT_RS22050 0.45 +0.4 502,594 - BPHYT_RS22050 0.46 -1.6 502,836 + BPHYT_RS22050 0.61 +0.2
Or see this region's nucleotide sequence