Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22030 and BPHYT_RS22035 overlap by 4 nucleotides BPHYT_RS22035 and BPHYT_RS22040 are separated by 1 nucleotides BPHYT_RS22040 and BPHYT_RS22045 are separated by 3 nucleotides
BPHYT_RS22030: BPHYT_RS22030 - peptide ABC transporter ATP-binding protein, at 497,961 to 498,986
_RS22030
BPHYT_RS22035: BPHYT_RS22035 - peptide ABC transporter ATP-binding protein, at 498,983 to 499,981
_RS22035
BPHYT_RS22040: BPHYT_RS22040 - peptide ABC transporter permease, at 499,983 to 500,897
_RS22040
BPHYT_RS22045: BPHYT_RS22045 - peptide ABC transporter permease, at 500,901 to 501,839
_RS22045
Position (kb) 498
499
500 Strain fitness (log2 ratio) -2
-1
0
1 at 498.200 kb on - strand, within BPHYT_RS22030 at 498.221 kb on - strand, within BPHYT_RS22030 at 498.263 kb on - strand, within BPHYT_RS22030 at 498.693 kb on - strand, within BPHYT_RS22030 at 498.986 kb on + strand at 498.986 kb on + strand at 498.986 kb on + strand at 498.987 kb on - strand at 498.987 kb on - strand at 498.987 kb on - strand at 500.041 kb on + strand at 500.219 kb on - strand, within BPHYT_RS22040 at 500.446 kb on + strand, within BPHYT_RS22040 at 500.447 kb on - strand, within BPHYT_RS22040 at 500.447 kb on - strand, within BPHYT_RS22040 at 500.459 kb on - strand, within BPHYT_RS22040 at 500.933 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 498,200 - BPHYT_RS22030 0.23 -0.3 498,221 - BPHYT_RS22030 0.25 -1.3 498,263 - BPHYT_RS22030 0.29 -0.0 498,693 - BPHYT_RS22030 0.71 -0.6 498,986 + +1.6 498,986 + -1.0 498,986 + -1.4 498,987 - -0.8 498,987 - +0.1 498,987 - +0.2 500,041 + -2.7 500,219 - BPHYT_RS22040 0.26 +0.6 500,446 + BPHYT_RS22040 0.51 -2.1 500,447 - BPHYT_RS22040 0.51 -0.8 500,447 - BPHYT_RS22040 0.51 +0.6 500,459 - BPHYT_RS22040 0.52 -0.2 500,933 - -2.4
Or see this region's nucleotide sequence