Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22025 and BPHYT_RS22030 are separated by 176 nucleotides BPHYT_RS22030 and BPHYT_RS22035 overlap by 4 nucleotides BPHYT_RS22035 and BPHYT_RS22040 are separated by 1 nucleotides
BPHYT_RS22025: BPHYT_RS22025 - porin, at 496,195 to 497,784
_RS22025
BPHYT_RS22030: BPHYT_RS22030 - peptide ABC transporter ATP-binding protein, at 497,961 to 498,986
_RS22030
BPHYT_RS22035: BPHYT_RS22035 - peptide ABC transporter ATP-binding protein, at 498,983 to 499,981
_RS22035
BPHYT_RS22040: BPHYT_RS22040 - peptide ABC transporter permease, at 499,983 to 500,897
_RS22040
Position (kb) 497
498
499 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 497.109 kb on - strand, within BPHYT_RS22025 at 497.148 kb on - strand, within BPHYT_RS22025 at 497.342 kb on + strand, within BPHYT_RS22025 at 497.375 kb on + strand, within BPHYT_RS22025 at 497.640 kb on + strand at 497.681 kb on + strand at 497.789 kb on + strand at 497.963 kb on - strand at 498.200 kb on - strand, within BPHYT_RS22030 at 498.221 kb on - strand, within BPHYT_RS22030 at 498.263 kb on - strand, within BPHYT_RS22030 at 498.693 kb on - strand, within BPHYT_RS22030 at 498.986 kb on + strand at 498.986 kb on + strand at 498.986 kb on + strand at 498.987 kb on - strand at 498.987 kb on - strand at 498.987 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 497,109 - BPHYT_RS22025 0.57 +0.4 497,148 - BPHYT_RS22025 0.60 -3.1 497,342 + BPHYT_RS22025 0.72 -0.9 497,375 + BPHYT_RS22025 0.74 +1.9 497,640 + -0.7 497,681 + +0.4 497,789 + +2.1 497,963 - +1.7 498,200 - BPHYT_RS22030 0.23 -0.3 498,221 - BPHYT_RS22030 0.25 -1.3 498,263 - BPHYT_RS22030 0.29 -0.0 498,693 - BPHYT_RS22030 0.71 -0.6 498,986 + +1.6 498,986 + -1.0 498,986 + -1.4 498,987 - -0.8 498,987 - +0.1 498,987 - +0.2
Or see this region's nucleotide sequence